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《浙江省第四届动物学博士与教授论坛、动物学与经济强省-浙江省动物学研究及发展战略研讨会论文摘要集》2017年
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miR-92a regulates coelomocytes apoptosis in sea cucumber Apostichopus japonicus via targeting Aj14-3-3ζ in vivo

Miao Lv  Huahui Chen  Yina Shao  Chenghua Li  Weiwei Zhang  Xuelin Zhao  Chunhua Jin  Jinbo Xiong  
【摘要】:miR-92 a,a well-documented oncogene,was previously found to be differentially expressed in diseased sea cucumber Apostichopus japonicus by high-throughput sequencing.In this study,we identified Aj14-3-3ζ as a novel target of miR-92 a in this species and investigated their regulatory roles in vivo.The negative expression profiles between miR-92 a and Aj14-3-3ζ protein were detected in both LPS-exposed primary coelomocytes and Vibrio splendidus-challenged sea cucumbers.Over-expression of miR-92 a by injection of miR-92 a agomir significantly depressed the mRNA and protein expression of Aj14-3-3ζ and promoted coelomocytes apoptosis with 5.04-fold increase in vivo,which was consistent with those from siRNA-mediated Aj14-3-3ζ knockdown assay.In contrast,miR-92 a antagomir significantly elevated the mRNA and protein expression of Aj14-3-3ζ and decreased coelomocytes apoptosis.Taken together,our result confirmed that miR-92 a is involved in apoptotic signaling pathway regulation perhaps via targeting Aj14-3-3ζ,in sea cucumbers,which will enhance our understanding of miR-92 a regulatory roles in sea cucumber pathogenesis.

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