Activation of mTOR Pathway Confers Adverse Outcome in Nonsmall Cell Lung Carcinoma

【摘要】：正Background:Dysregulation of the mammalian target of rapamycin(mTOR) pathway has been shown to contribute to tumorigenesis.This study explored protein expression profiles of mTOR pathway and the relationship with prognosis in patients with nonsmall cell lung carcinoma(NSCLC). Methods:The protein expression profiles of mTOR/phosphorylated mTOR(p-)mTOR,phosphoinositide-dependent kinase 1(PDK1)/ p-PDK1,p-Akt1,and P70 ribosomal protein S6 kinase(P70S6K)/ p-P70S6K were determined via immunohistochemical staining assay.The clinical prognostic values of both single and combined protein expression were investigated with univariate and multivariate survival analysis.The correlations between these biomarkers were analyzed using Spearman' s rank test. Results:Overall survival in NSCLC patients was significantly shorter in cases with positive phenotype for p-mTOR, P-PDK1 and p-P70S6K(all pP Conclusions:This study provides clinical evidences that activated components of mTOR pathway,not total protein,are predictors of poor prognosis in NSCLC.Moreover,evaluating protein expression profiles of these molecules might be a new strategy for individual therapy in subjects with NSCLC.