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《2016全国中西医结合妇产科研究进展学术研讨会暨2016年第一届江浙沪中西医结合妇产科高峰论坛论文及摘要集》2016年
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Effects of Intraperitoneal Injection of Cisplatin on Mouse Ovary-related Gene Expression Profile

Ai Hao  Xue xiaoou  
【摘要】:Objective:DNA microarray technology was used to investigate the effects of intraperitoneal injection of cisplatin on mouse ovary-related gene expression,and explore the mechanisms of cisplatin-induced ovarian injury.Methods:Chemotherapy-induced mouse ovarian dysfunction model was established.The one-step method was used to extract total RNA from mouse ovarian tissues for both cisplatin treatment group and control group.Mouse ovarian cDNA probes were obtained for both groups by reverse transcription and labeled with Cy3 and Cy5 fluorescence markers,respectively before hybridizing with cDNA microarray and scanning with a laser scanner.Then,computer software was used for image analysis,standardization,ratio analysis and cluster analysis.Results:Mouse ovarian tissue gene expression profiles were analyzed for both cisplatin treatment group and control group.Of the 109 differentially expressed genes identified,19 were under-expressed genes and 90 were over-expressed genes.Conclusion:In this study,a large number of differentially expressed genes are screened from mouse ovarian tissues after cisplatin treatment,suggesting that these genes may participate in the occurrence and development of cisplatin-induced ovarian injuries.These results have provided the basis for diagnosis and treatment of chemotherapeutic drug-induced ovarian dysfunction.

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