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《2017中国长三角遗传学大会会议手册》2017年
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Bioinformatics analysis of Pleurotus ostreatus laccase genes and their expression in the cotton-straw solid medium

Xiaoyu Jiao  Guoqing Li  Yan Wang  Fan Nie  Xi Cheng  Muhammad Abdullah  Yi Lin  Yongping Cai  
【摘要】:Fungal laccases play important roles in the degradation of lignocellulose.Although some PoLACs were reported in several studies,no comprehensive bioinformatics study of the LAC family in Pleurotus ostreatus has been reported.In this study,an exhaustive screening of the Pleurotus ostreatu genome was performed.We identified 12 laccases genes in the whole genome sequence of Pleurotus ostreatus,which is same as the gene sequence that annotated as laccase on P.ostreatus genome.Altogether their physical characteristics,gene distribution,phylogenic relationships,gene structure,conserved motif and promoter sequence were also analyzed.The results showed that all of the 12 laccases have the signal peptide and no transmembrane region,indicated that these laccases belong to the secretory protease.The 12 PoLACs were unevenly distributed on 5 scaffolds and clustered on scaffold 6 and scaffold 11.The analysis of multiple sequence alignment revealed that all predicted amino acids sequences contained complete laccase signature sequences L1-L4 except PoLAC5,PoLACll,and PoLAC12.The substrate binding loops of PoLAC2 and PoLAC5 were significantly different from those of other sequences.Combined with the above analysis,the results suggesting that PoLAC5 is not a typical laccase,and may be a ferroxidase in the family of multicopper oxidases(MCOs).In addition to the core promoter elements(TATA-box and CAAT-box) of eukaryotes,there are many other putative cis-acting elements(MRE,XRE,ARE,HSE,NIT and STRE elements,etc.).Subsequently,the expression patterns of 12 PoLac genes at different developmental stages and under different culture substrates were analyzed.The distinctly higher expression levels observed for PoLac2 genes during the initial stages of mycelium period indicated that PoLac2 may play a major role in the degradation of lignin.With the formation of primordia and fruiting bodies,the expression of PoLacl2 increased significantly,suggesting that PoLacl2 may be involved in the process of fruiting body formation.The results of this study provide a basis for further analysis of PoLac gene family putative functions.

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