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《2017中国长三角遗传学大会会议手册》2017年
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Karyotype analysis of woodland strawberry(Fragaria vesca)with Oligopaint FISH probes

Manman Qu  Kunpeng Li  Yanli Han  Lei Chen  Zongyun Li  Yonghua Han  
【摘要】:Chromosome identification is critical for many aspects of cytogenetic research.However,for Fragaria vesca,a model species in Fragaria genus,definitive identification of the individual chromosomes is almost impossible because of their small size and high similarity.Here we demonstrated that bulked oligonucleotide(oligo) probes can be used as chromosome-specific DNA markers for chromosome identification in F.vesca.Oligos specific to an entire chromosome of F.vesca were identified and synthesized as a library.In all,we synthesized 6 oligo libraries corresponding to 6 pseudochromosomes in the genome assembly of F.vesca,respectively.These libraries were amplified and labeled as probes for fluorescence in situ hybridization(FISH).Two rounds of multicolor FISH analysis were sequentially conducted on the same metaphase cells with each round including three probe libraries,which permitted simultaneous identification of all chromosomes of F.vesca.Moreover,45 S and 5 S rDNA were mapped to chromosomes 1,2 and 7,respectively.A standardized karyotype of somatic metaphase chromosomes was constructed.This is the first FISH-based cytogenetic karyotype of F.vesca.Our study will serve as a basis for future comparative cytogenetic research through cross-species chromosome painting using bulked oligo probes and will facilitate applied breeding technologies that rely upon identification of chromosomes in the genus Fragaria.

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