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《2017中国长三角遗传学大会会议手册》2017年
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Molecular Cloning and Expression Analysis of Potassium Transporter Gene,MaHAK5,in Mulberry

Chen Dan-dan  Zhao Wei-Guo  
【摘要】:Potassium(K +) is one of the many elements necessary for plant growth and development,and is the only one that plants need to have a higher concentration of cation.In order to cope with the low potassium stress,the plant evolves a series of complex signal regulation pathways.HAK5 is an important high-affinity potassium transporter,which is mainly responsible for potassium uptake under low potassium conditions,and low potassium stress can significantly induce transcription of HAK5.In this experiment,the complete reading frame(ORF) of the gene was cloned from the young leaves of mulberry Yu 71-1,named MaHAK5.Sequence analysis showed that the MaHAK5 is 2625 bp long,It's open reading frame(ORF) is of 2478 bp,and encoding 828 amino acids with a predicted molecular weight of 92.17 kD and an isoelectric point of 8.37.Phylogenetic analysis based on MaHAK5 with other species showed that mulberry had a closer relationship with Chuansang(Moras notabilis XP_010112002.1),Jujube(Ziziphus jujuba XP_015898221.1),Fragaria vesca(Fragaria vesca subsp.Vesca XP_004299195.2).Drought,high salt and low temperature stress could affect the expression level of MaHAK5 gene in mulberry,and the expression level of MaHAK5 gene was increased and the physiological and biochemical indicators were affected by low potassium treatment.
【作者单位】:Jiangsu University of Science and technology
【分类号】:S888.2

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