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《2017中国长三角遗传学大会会议手册》2017年
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Precision Plant Breeding Using Genome Editing Technologies

Caixia Gao  
【摘要】:Genome editing can accelerate plant breeding by allowing the introduction of precise and predictable modifications directly in an elite background.The most promising utilization of both the CRISPR/Cas9 system and TALENs can be used to generate targeted genome modifications including mutations,insertions,replacements and chromosome rearrangements.We developed simple and efficient genome editing approaches in which wheat plants are regenerated from callus cells transiently expressed with CRISPR/Cas9 reagents introduced as DNA,RNA or RNP.The effectiveness of the three methods in yielding specifically targeted,transgene-free mutants in TO generation was validated using six different wheat genes.Recently,we established a plant base editing protocol suitable for introducing targeted point mutations to wheat,rice and maize genomes.This approach will not only technologically advance plant genome engineering,but may also provide better solution for social acceptance of genome-edited crops as they do not require a donor DNA template or chromosomal cleavage.These approaches may be widely applicable for producing genome edited crop plants and has a good prospect of being commercialized.

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