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Characterization of asexual flocculation of fission yeast cell resulted from deletion of ribosomal protein L32

李荣鹏  Zhonghua Liu  Qing Dong  Lezhi Bian  Xuesong Li  Sheng Yuan'  
【摘要】:Recently we reported that deleting either of the two rpl32 paralogous genes resulted in a non-sexual flocculation in fission yeast.This study explores that this non-sexual flocculation is an adhesin-mediated process:(1) the transcription level of several members of Mam3/Map4 family adhesins,SPAC186.01,SPAC947.04,SPAC977.07 c and SPBC359.04 c,as well as a adhesin Floll like protein are up-regulated in rpl32-lA and rpl32-2A cells;(2) this non-sexual flocculation of rpl32-lA and rpl32-2A cells was eliminated by heating or enzyme digestion;(3) this non-sexual flocculation of rpl32-lA and rpl32-2A cells was enhanced by Ca~(2+) and some other divalent metal ions,as stabilizers of adhesin active confirmation;(4) this non-sexual flocculation of rpl32-1Δ and rpl32-2A cells was competitively inhibited by either of glucose,galactose or mannose,rather than only by galactose as reported previously.Apparently though different adhesin genes are expressed selectively upon some particular physiological or environment conditions their functions are the same and are interchangeable.Furthermore,this study first report that these non-sexual flocculating fission yeast cells exhibit a thicker cell wall,more wall proteins content and smear glycosylated wall proteins,and more cell wall polysaccharide content and adhesin binding sugar residues,glucose,mannose and glacotose,which reflect the wall features of flocculating cells for recognition and connection between cells.

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