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《中国遗传学会第九次全国会员代表大会暨学术研讨会论文摘要汇编(2009-2013)》 2013年
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Silencing of soybean Acyl-ACP thioesterase B (GmFatB) increases palmitoleic acid accumulation in soybean oil

XUE Jin-Ai  MAO Xue  YANG Zhi-Rong  JIA Xiao-Yun  WU Yong-Mei  GAO Xiu-Qing  HUANG Chun-Guo  ZHANG Li  WANG Ji-Ping  LI Run-Zhi  
【摘要】:正Acyl-acyl carrier protein(ACP) thioesterases are enzymes that terminate the intraplastidial fatty acid synthesis in plants by hydrolyzing the acyl-ACP intermediates and releaseing free fatty acids to be incorporated into glycerolipid in the endoplasmic reticulum(ER) These enzymes are classified in two families,FatA and FatB,which differ in amino acid sequence and substrate specificity.We previously isolated and identified both FatA and FatB from a high palmitoleic acid accumulator plant,indicating that FatB has a high preference towards palmitoyl-ACP and selectively transport acyl-ACP 16:0 from plastid to cytosol.In this work,we characterized five GmFatBs from soybean by bioinformatics tools and experimental analysis in detail.Their high expressions limited palmitoleic acid synthesis in soybean seeds.Small RNA silencing technique was employed to down-regulated all five GmFatB expressions in seeds with a goal to increase palmitoleic acid synthesis for healthy oil production.Compared to the non-transgenic seeds,the transgenic soybean developing seeds exhibited 67%reduction of all five GmFatB transcripts.Accordingly,acyl-ACP 16:0 content in plastid was enhanced by 39% Therefore,more acyl-ACP 16:0 substrates were provided for acy1-ACP-A9 desaturase to produce high level of acyl-ACP palmitoleic acid(C16:1△9) in the seeds.The engineered soybean oil with 10%palmitoleic acid was obtained(Fig 1),and those transgenic plants showed no changes in other phenotypes.Such engineered soybean oil could be used for commercial production of this unusual fatty acid for human food,health and industry.The present results demonstrate that high-valued unusual fatty acids such as palmitoleic acid can be synthesized and accumulated in common oil seeds by metabolic engineering approach.

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