Determination of nine volatile N-nitrosamines in tobacco and smokeless tobacco products by dispersive solid-phase extraction with gas chromatography and tandem mass spectrometry
【摘要】：A method was developed for the determination of nine volatile N-nitrosamines in tobacco and smokeless tobacco products. The targets are N-nitrosodimethylamine, Nnitrosopyrrolidine, N-nitrosopiperidine, N-nitrosomorpholine, N-nitrosoethylmethylamine,N-nitrosodiethylamine, N-nitrosodipropylamine, N-nitrosobuylmethylmine, and Nnitrosodibutylamine. The samples were treated by dispersive solid-phase extraction using 1 g of primary secondary amine and 0.5 g of carbon and then analyzed by gas chromatography with tandem mass spectrometry with an electron impact ion source. The recoveries for the targets ranged from 84 to 118%, with 16% relative standard deviations at three spiking levels of 0.5, 1.25, and 2.5 ng/g. The limits of detection ranged from 0.03 to 0.15 ng/g.With the use of the proposed method, we detected the presence of six nitrosamines in the range of 0.4–30.7 ng/g. The study demonstrated that the method could be used as a rapid,convenient, and high-throughput method for N-nitrosamines analysis in tobacco matrix.