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《中国烟草学会学术年会优秀论文集》2017年
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Sequencing and phylogenetic analysis of tobacco virus 2, a polerovirus from Nicotiana tabacum

Benguo Zhou  Fang Wang  Xuesong Zhang  Lina Zhang  Huafeng Lin  
【摘要】:The complete genome of a new virus, provisionally named tobacco virus 2(TV2), was determined and identified from leaves of tobacco(Nicotiana tabacum) exhibiting leaf mosaic, yellowing, and deformity, in Anhui Province, China. The genome sequence of TV2 comprises 5,979 nucleotides, with 87% nucleotide sequence identity with Potato leafroll virus(PLRV). Its genome organization is similar to PLRV, containing six open reading frames(ORFs) that potentially encode proteins with putative functions in cell-to-cell movement and suppression of RNA silencing. Phylogenetic analysis of the nucleotide sequence placed TV2 alongside members of the genus Polerovirus in the family Luteoviridae. To the best our knowledge, this study is the first report of complete genome sequence of a new Polerovirus identified in tobacco.

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