收藏本站
《中国烟草学会学术年会优秀论文集》2017年
收藏 | 手机打开
二维码
手机客户端打开本文

Analysis of Nicotiana tabacum PIN genes identifies NtPIN4 as a key regulator of axillary bud growth

Xiaodong Xie  Guangyong Qin  Ping Si  Zhaopeng Luo  Junping Gao  Xia Chen  Jianfeng Zhang  Pan Wei  Qingyou Xia  Fucheng Lin  Jun Yang  
【摘要】:The plant-specific PIN-FORMED(PIN) auxin efflux proteins have been well characterized in many plant species, where they are crucial in the regulation of auxin transport in various aspects of plant development. However, little is known about the exact roles of the PIN genes during plant development in Nicotiana species. This study investigated the PIN genes in tobacco(Nicotiana tabacum) and in two ancestral species(Nicotiana sylvestris and Nicotiana tomentosiformis). Genome-wide analysis of the N. tabacum genome identified 20 genes of the PIN family. An in-depth phylogenetic analysis of the PIN genes of N. tabacum, N. sylvestris and N. tomentosiformis was conducted.Nt PIN4 expression was strongly induced by the application of exogenous indole-3-acetic acid(IAA), but was downregulated by the application of ABA,a strigolactone analogue, and cytokinin, as well as by decapitation treatments,suggesting that the Nt PIN4 expression level is likely positively regulated by auxin. Expression analysis indicated that NtP IN4 was highly expressed in tobacco stems and shoots, which was further validated through analysis of the activity of the Nt PIN4 promoter. We used CRISPR-Cas9 technology to generate mutants for Nt PIN4 and observed that both T_0 and T_1 plants had a significantly increased axillary bud growth phenotype, as compared with the wild-type plants. Therefore, Nt PIN4 offers an opportunity for studying auxin-dependent branching processes.

【相似文献】
中国期刊全文数据库 前10条
1 刘卫群,饶学明,潘继承,周海梦;Kinetics of Inhibition of Polyphenol Oxidase Obtained from Tobacco Nicotiana Tobacum[J];Tsinghua Science and Technology;2004年01期
2 ;Nicotiana ovule extracts induce nuclear reconstitution of demembranated Xenopus sperm in cell-free system[J];Chinese Science Bulletin;2002年04期
3 G. Sandmann;T. Gerjets;M. Sandmann;;Metabolic Engineering of Carotenoid Synthesis: Development of a Production System with Nicotiana glauca and Improvement of the Nutritional Value of Potato[J];分子植物育种;2007年02期
4 Hendrik Wünsche;Ian T. Baldwin;;Silencing NOA1 Elevates Herbivory-Induced Jasmonic Acid Accumulation and Compromises Most of the Carbon-Based Defense Metabolites in Nicotiana attenuata[J];Journal of Integrative Plant Biology;2011年08期
5 Celia Diezel;Silke Allmann;Ian T.Baldwin;;Mechanisms of Optimal Defense Patterns in Nicotiana attenuata: Flowering Attenuates Herbivory-elicited Ethylene and Jasmonate Signaling[J];Journal of Integrative Plant Biology;2011年12期
6 Lei Wang;Jianqiang Wu;;The Essential Role of Jasmonic Acid in Plant-Herbivore Interactions-Using the Wild Tobacco Nicotiana attenuata as a Model[J];Journal of Genetics and Genomics;2013年12期
7 崔晓峰,李云琴,胡东维,周雪平;Science Letters:Expression of a begomoviral DNAβ gene in transgenic Nicotiana plants induced abnormal cell division[J];Journal of Zhejiang University Science;2005年02期
8 Bo SUN;Fen ZHANG;Guo-jun ZHOU;Guo-hai CHU;Fang-fang HUANG;Qiao-mei WANG;Li-feng JIN;Fu-cheng LIN;Jun YANG;;Genetic variation in alkaloid accumulation in leaves of Nicotiana[J];Journal of Zhejiang University-Science B(Biomedicine & Biotechnology);2013年12期
9 ;Functional Analysis of DHS by Virus-induced Gene Silencing in Nicotiana benthamiana[J];分子植物育种;2007年02期
10 曲冠证;郑唐春;杜恒瑞;臧丽娜;夏德安;;烟草Nicotiana glauca遗传瘤的诱导[J];安徽农业大学学报;2012年03期
中国重要会议论文全文数据库 前10条
1 ;Interference between Tobacco necrosis virus and Turnip crinkle virus in Nicotiana benthamiana[A];中国植物病理学会2008年学术年会论文集[C];2008年
2 ;Evolution of the Resistance against TMV in Nicotiana spp.[A];第十三届全国植物基因组学大会论文集[C];2012年
3 ;Molecular cloning and functional characterization of a putative sulfite oxidase(SO) ortholog from Nicotiana benthamiana[A];2011全国植物生物学研讨会论文集[C];2011年
4 ;Specific moss is the key nurse plant for reintroduction success of rare and endangered Primulina tabacum Hance[A];第五届中国青年生态学工作者学术研讨会论文集[C];2008年
5 ;RNA-Dependent RNA Polymerase 1 from Nicotiana tabacum Suppresses RNA Silencing and Enhances Viral Infection in Nicotiana benthamiana[A];中国植物病理学会2010年学术年会论文集[C];2010年
6 ;Moss is a key nursing plant for reintroduction success of an endangered herb,Primulina tabacum Hance[A];第三届全国植物生态学前沿论坛第三届全国克隆植物生态学研讨会论文摘要汇编[C];2009年
7 ;An Efficient System to Detect Protein Ubiquitination by Argoinfiltration in Nicotiana Benthamiana[A];植物分子生物学与现代农业——全国植物生物学研讨会论文摘要集[C];2010年
8 ;Genetic Diversity and Evolution of Tobacco(Nicotiana tabacum L.) Germplasm Resources Based on ISSR and SRAP Markers[A];2009整合植物生物学研讨会摘要集[C];2009年
9 ;The Suppressors of RNA Silencing Encoded by Lily mottle virus[A];中国植物病理学会2008年学术年会论文集[C];2008年
10 Christian Hettenhausen;Ian T.Baldwin;;Silencing Nicotiana attenuata calcium-dependent protein kinases,CDPK4 and CDPK5,strongly upregulates wound- and herbivory-induced jasmonic acid accumulations[A];第十三届全国植物基因组学大会论文集[C];2012年
中国博士学位论文全文数据库 前1条
1 Sandra Pérez Alvarez;[D];浙江大学;2005年
中国硕士学位论文全文数据库 前2条
1 毛兰兰;烟草属(Nicotiana)抗病基因VIGS沉默系统的构建[D];华中农业大学;2011年
2 王永亮;烟草本塞姆氏(Nicotiana Benthamiana)中DREB转录因子的克隆及功能分析[D];河南农业大学;2006年
 快捷付款方式  订购知网充值卡  订购热线  帮助中心
  • 400-819-9993
  • 010-62791813
  • 010-62985026