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Mosquitoes of shanghai,china:The distribution and ecology Associations of Arboviral disease

WUYA-ling  WEI Jian-chao  HUANG Ke-he  SHI-Kun  LI Lun-Feng  MA Gai-Ni  LI Qian-qian  LI Bei-bei  LIU Ke  SHAO Dong-hua  QIU Ya-feng  MA Zhi-yong  
【摘要】:Objective:Shanghai province is located in east china,This area has a northern subtropical monsoon climate,with an average annual temperature about 15 ℃.several other viruses have been isolated from human patients and from mosquitoes collected in shanghai Province.The present study was undertaken in four urban sites of shanghai district during June 2014 and August 2014.This project was to describe distribution in mosquito species abundance and ecology about arbovirus activity in the mosquito populations.Methods:Mosquitoes were collected in four urban sites of shanghai district during June 2014 and August 2014.Collected mosquitoes were immediately transported from the field sites to the laboratory and killed with cold by placing them at-20℃ for 30 min.Specimens were then identified to species and sex,and counted on a chill table.Following identification to species,females were sorted into pools of up to 100 specimens according to species,date,location,and trap type.Pools of mosquitoes were stored on liquid nitrogen until processed for arboviruses at the laboratory of the Shanghai veterinary research Institute,female mosquitoes were identified to the species level using morphologic characteristics and subsequently stored in liquid nitrogen.The male mosquitoes were discarded.Results:A total of 49,804 female mosquitoes comprised of 11 species representing four genera were collected using the UVT mosquito traps in pigpen near the cities of Minhang Fengxian and Pudong Jiading district in Shanghai China.Of the total number,including 4 species of Culex,2 species of Anopheles,and 1 species of Armigeres.The predominant species were Cx.tritaeniorhynchus(57.9%of the total;28,856/49,804),and midges(28.56%;14225/49,804),and Anopheles sinensis(1.58%;789/49,804),1.38%were Culex bitaeniorhynchus(688/49,804).A total of 13 virus strains were isolated,which Include 6 strains of Japanese encephalitis virus belonging to the Genotype GI.

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