SNPs of GH Gene Characterized by PCR-SSCP Analysis and Search for Their Associations with Milk Yield in Goat
【摘要】：正Single nucleotide polymorphisms (SNPs) characterized by PCR-SSCP analysis in exon V of growth hormone (GH) gene for 180 goats from five goat breeds were investigated and their associations with milk yield of Xinong Saanen dairy goats were searched. The results showed that four SSCP patterns were observed in goat. In reference to goat GH gene sequence (GenBank No.D00476) as a control, SSCP pattern sequences characterized 14 SNPs, among which 4 codon mutations changed no amino acid variations. The establishment of relationships between SSCP patterns and SNPs and milk performance from Xinong Saanen dairy goats was attempted by a general mixed linear model. Significant statistical relationship between 3 SSCP patterns and milk yields was observed (P0.05), and significant association of 4 SNPs with milk production traits was preliminary to be found (P0.05 and P0.01). Hence, we drew a preliminary conclusion that 4 SNPs characterized by PCR-SSCP patterns for exon V of GH gene exerted significant effects on milk performance, could be considered as molecular marker in MAS.