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《第二届中国临床分子诊断大会论文集》2019年
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Microarray profiling of circRNA expression in radiation-treated A549 cells and bioinformatics analysis of radiation-related circRNA-miRNA networks

Ting Zhang  Dong-ming Wu  Shi-hua Deng  Rong Han  Teng Liu  Jing Li  Ying Xu  
【摘要】:Objective: With the development of new biochemical and computational methods, circular RNAs(circRNAs) have been identified as microRNA sponges, involved in many diseases, especially cancers. The aim of this study is to investigate the role of circRNAs in irradiated A549 lung cancer cell line. Methods: High-throughput sequencing was used to identify circRNA expression profiles in irradiated and non-irradiated A549 cells. Quantitative real-time PCR(qRT-PCR) was performed to confirm the reliability of high-throughput RNA sequencing. Bioinformatics analyses were used to examine the potential functions of circRNAs. Results: The microarray data showed that 1875 circRNA targets in the A549 cells were differentially expressed in response to irradiation. There were 30 circRNAs upregulated and 37 circRNAs downregulated significantly in irradiation-treated A549 cells(fold change ≥ 2.0 and P 0.05). The top five upregulated and downregulated circRNAs were successfully verified by qRT-PCR. Gene ontology(GO) and Kyoto encyclopedia of genes and genomes(KEGG) pathway revealed that differentially expressed circRNAs might play an important role in irradiation reaction and biological process. CircRNA-miRNA co-expression network suggested that circRNA_0002174 and circRNA_0036627 were the most interesting circRNAs deserved further study. Conclusions: Our study is the first to reveal the profile of differentially expressed circRNAs in response to irradiation in A549 cells. These results provide a new perspective to elucidate the molecular mechanisms by which A549 cells respond to radiation and a basis for further investigating the potential application of circRNAs in lung cancer therapeutic approaches.
【作者单位】:Clinical Laboratory, The First Affiliated Hospital of Chengdu Medical College
【分类号】:R734.2;R730.55

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