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Transcriptome analysis reveals candidate genes involved in pathogenicity in Sclerotinia sclerotiorum

Lv Xingming  Liu Jinliang  Zhang Yanhua  Zhang Xianghui  Pan Hongyu  
【摘要】:Sclerotinia sclerotiorum is an important plant pathogenic fungus which has a wide hostrange and can infect more than 400 plant species,including many important crop plants.In previous study,a transcriptional factor Ss-Nsd1 gene from S.sclerotiorum was cloned and the function of Ss-Nsd1 was characterized by gene knockout.The Ss-nsdl deletion mutants were defective in compound appressorium formation,which is a crucial step for phytopathogenic fungi to initiate infection of their hosts due to the penetration of the plant cuticle,meanwhile the mutants lost the ability to invade its host plant.In this study,the Ss-nsdl deletion mutants and wild type strains were used to analyse differentially expressed genes(DEGs) by RNA Sequencing.The partial candidate genes of compound appressorium formation and pathogenecity were functional analyzed.The RNA-seq results revealed 2470 DEGs(Log2FC1 or -1,FDR 0.01) enriched in 1789 GO terms.KEGG analysis indicated that the up-regulated genes and down-regulated genes were significantly enriched in 8 pathways and 11 pathways(P0.05),respectively.Based on these results,virulent genes of S.sclerotiorum were deeply excavated and the expression levels of DEGs were verified by fluorescence quantitative PCR which showed consistent results with the sequencing analysis outcomes.The role of these virulent genes were under investigation.

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