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SsFDH1,a formaldehyde dehydrogenase,regulates the sclerotial development and pathogenicity in Sclerotinia sclerotiorum

Zhu Genglin  Li Jingtao  Lv Xingming  Zhang Xianghui  Liu Jinliang  Zhang Yanhua  Pan Hongyu  
【摘要】:Sclerotinia sclerotiorum(Lib.) de Bary is notorious phytopathogenic fungus with broad host ranges.Glutathione-dependent formaldehyde dehydrogenases(GD-FDHs) represent a ubiquitous class of enzymes in both prokaryotes and eukaryotes,but the functions of FDHs in necrotrophic plant pathogen Sclerotinia sclerotiorum have not been characterized.In this study,we functionally characterized a GD-FDH orthologous protein SsFDH1(SS1G_10135)in S.sclerotiorum.SsFDH1 was firstly identified as an interacting protein of our previously characterized GATA transcription factor SsNsdl,which regulates asexual-sexual development and appressoria formation in S.sclerotiorum.Consistent to its predicted biochemical function as one of the FDHs in formaldehyde detoxification,SsFDH1 deletion mutants(△SsFDH1-KOs) were lethal when grown in formaldehyde-containing medium and showed sensitivity to exogenous nitrosative stress as well.Morphologically,△SsFDH1-KOs showed altered sclerotial development,reduced production of compound appressoria,as well as increased sensitivity to different osmotic and oxidative stresses.Meanwhile,the △SsFDH1-KOs exhibited attenuated virulence on both healthy and wounded common bean leaves.Compared with the previously generated △SsNsd-KOs,△SsFDH1-KOs produced smaller-sized sclerotium,but showed similar attenuated pathogenicity.Additionally,we found that the expression levels of both SsNsd1 and SsFDH1 displayed spatial and temporal synchronization as revealed by quantitative real-time PCR(qRT-PCR) analysis in different developmental stages.Taken together,our results suggested that SsFDH1,as a SsNsd1-interating protein,might function jointly with SsNsd1 in vegetative development and pathogenicity in S.sclerotiorum.

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