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The research on transcription factor SsMCM1 and its interacting proteins in Sclerotinia sclerotiorum

Zhang Bowen  Xu Tingtao  Liu Ling  Zhu Genglin  Lv Xingming  Liu Jinliang  Zhang Xianghui  Zhang Yanhua  Pan Hongyu  
【摘要】:Sclerotinia sclerotiorum(Lib.) de Bary is a challenging agricultural pathogen for management,causing large global economic losses annually.In this study,a MADS-box transcriptional factor SsMCM1 was cloned from S.sclerotiorum,then SsMCM1 function was investigated using RNA interference.Our results demonstrated that the transcription factor SsMCMl was involved in S.sclerotiorum growth,development and virulence.Yeast two-hybrid and Bimolecular Fluorescent Complementary was been applied to identify proteins that physically associate with SsMCMl in S.sclerotiorum.Twelve putative SsMCMl interacting proteins were identified.SsIP37 contains the Pmp3 family domain;SsIP43 contains the PGM3 family domain;SsIP44 contains the Mpvl7-PMP22 domain;SsIP86 contains the Catalase domain;SsIP98 contains the Cyclin domain;SsIP106 contains the RGL11 domain.The other interacting proteins function are unknown.SsCdc28(SSIG_02296) was cloned which highly homologous to yeast Cdc28.Yeast twohybrid assays indicate that Cdc28 physically interacts with SsIP86.SsIP99(SS1 G_07136) contained an STE domain at the N terminus and a C2 H2 zinc finger domain at the C terminus,termed as SsSte12,the putative downstream transcription factor of MAPK pathway in S.sclerotiorum.Silencing SsSte 12 resulted in phenotypes of delayed vegetative growth,reduced size of sclerotia,and fewer appressoria formation.Consequently,the SsSte12 RNAi mutants showed attenuated pathogenicity on the host plants due to the defect compound appressorium.Moreover,the transcription factor SsMCM1 and its interacting proteins might play a critical role in the regulation of the genes encoding these traits in S.sclerotiorum.

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