收藏本站
《中国神经科学学会第十二届全国学术会议论文集》2017年
收藏 | 手机打开
二维码
手机客户端打开本文

Transplantation of NT-3 delivery acellular optic nerve scaffold to improve motor function in transected rat spinal cord

Ge Li  Jia-Hui Sun  Yuan-Shan Zeng  
【摘要】:Objective To generate a bioactive scaffold with line-shaped tubes in vitro and investigate whether the scaffold transplanted into the injury site could promote nerve fiber regeneration and functional recovery in rat spinal cord transected completely. Methods By using aseptic decellular, DNAase hydrolysis and freeze-drying techniques, natural optic nerve was removed all cell component and coated with neurotrophin-3(NT-3)/fibroin complex at the surface to fabricate a NT-3 delivery acellular optic nerve scaffold. Then, dorsal root ganglion(DRG) was seeded on the surface of NT-3 delivery acellular optic nerve scaffold in vitro to observe the effect of the scaffold on DRG neurite growth, and the scaffold were transplanted into the injury site of spinal cord injury of rat. Results Fresh normal optic nerve of longitudinal section was stained by HE showing high-density tubes with optic nerve fibers. By using aseptic decellular technique and freeze-drying technique, the NT-3 delivery acellular optic nerve scaffold still maintained its natural 3 dimension(3 D) structure of extracellular matrix. Circle-shaped pipelines made of perineurium-like extracellular matrix in optic nerve of transverse section could be obseved and there were line-shaped tubes with interconnections in optic nerve of longitudinal section after all cell component was removed. These results were further conformed under a scanning electron microscope. The present study proved that cell component had been removed by the results below;1.Cell nuclei were invisible by Hoechst33342 staining. 2. DNA electrophoresis showed that residual DNA fragments were less than 200 bp. 3. DNA quantification showed 50 ng per mg scaffold dry weight. Therefore, cells seeded in the scaffold could grow without nucleic acid residue effect. Then, after DRG was seeded on the scaffold in vitro, we found that DRG derived NF200 positive neurites could grow a long distance in the surface of the scaffold. Furthermore, two months later after the scaffold transplanted, a lot of regenerating nerve fibers were observed in the injury/graft site of spinal cord and the motor function of paralysis hindlimbs was improved with higher BBB score and favorable cortical motor evoked potential. Conclusion The present findings suggest that NT-3 delivery acellular optic nerve scaffold can promote long distance growth of DRG neurite in vitro and may improve motor function of spinal cord transected completely by regenerating nerve fibers in vivo.

【相似文献】
中国期刊全文数据库 前10条
1 LEE Jun-Hee;PARK Su-A;PARK KoEun;KIM Jae-Hyun;KIM Kyung-Shik;LEE Jihye;Kim WanDoo;;Fabrication and characterization of 3D scaffold using 3D plotting system[J];Chinese Science Bulletin;2010年01期
2 ;Design,fabrication and application of tissue engineering used cells scaffold[J];Chinese Journal of Biomedical Engineering(English Edition);2001年02期
3 刘雷;唐康来;杨柳;简月奎;李起鸿;;Study of biological safety of scaffold material with heterologous deproteinized bone[J];Chinese Journal of Traumatology;2006年04期
4 潘勇;Fabrication of a Mood vessel scaffold with a combined polymer for tissue enginerring[J];China Medical Abstracts;2003年02期
5 刘雷;裴福兴;周宗科;李起鸿;;Cellular compatibility of improved scaffold material with deproteinized heterogeneous bone[J];Chinese Journal of Traumatology;2007年04期
6 ;The Use of Zein and Shuanghuangbu for Periodontal Tissue Engineering[J];International Journal of Oral Science;2010年03期
7 张晓欣;ZHANG Jiayin;施斌;;Mesoporous Bioglass/Silk Fibroin Scaffolds as a Drug Delivery System: Fabrication, Drug Loading and Release in vitro and Repair Calvarial Defects in vivo[J];Journal of Wuhan University of Technology(Materials Science Edition);2014年02期
8 LEE Seung-Jae;LEE In Hwan;PARK Jeong Hun;GWAK So-Jung;RHIE Jong-Won;CHO Dong-Woo;KO Tae Jo;KIM Dong Sung;;Development of a hybrid scaffold and a bioreactor for cartilage regeneration[J];Chinese Science Bulletin;2009年19期
9 漆小鹏;叶建东;;Microstructure and Mechanical Properties of Calcium Phosphate Cement/Gelatine Composite Scaffold with Oriented Pore Structure for Bone Tissue Engineering[J];Journal of Wuhan University of Technology(Materials Science Edition);2012年01期
10 ;Heart,cardiovalvula,vascular disorder[J];China Medical Abstracts(Surgery);2008年01期
中国重要会议论文全文数据库 前10条
1 ;Mechanical responses of osteoblastS in hard scaffold subjected direct compression during bone tissue engineering[A];第八届全国生物力学学术会议论文集[C];2006年
2 Wang Qingbo;Guo Wei;Zhang Chunlei;Feng Guoying;Zhang Luping;Huang Fei;;Comparative study of different methods for preparing decellularized nerve scaffold[A];中国解剖学会2013年年会论文文摘汇编[C];2013年
3 Zhongjie Lu;Sheng Yan;Jun Pan;Shusen Zheng;;Decelluarized mouse liver scaffold can effeciently induce foxp3 espression in cd4+cd25-T cells[A];2013中国器官移植大会论文汇编[C];2013年
4 祝小雷;来鲁华;;A Novel Method for Enzyme Design[A];第二届全国“跨学科蛋白质研究”学术讨论会论文集[C];2008年
5 Qiang-cheng Zeng;Yong Guo;;Cardiac tissue engineering scaffold of Small intestinal submucosa[A];第十届全国生物力学学术会议暨第十二届全国生物流变学学术会议论文摘要汇编[C];2012年
6 Yong Zheng;Cunjie Zhang;Mohamed A.Soliman;Rick Bagshaw;Adrian Pasculescu;Lorne Taylor;Anna Y.Dai;Tony Pawson;;Regulation of protein signaling networks by the scaffold protein Shcl in time and space[A];第七届全国医学生物化学与分子生物学和第四届全国临床应用生物化学与分子生物学联合学术研讨会暨医学生化分会会员代表大会论文集[C];2011年
7 ;Determining the Molecular Basis for the pH-dependent Interaction between the PDZ of Human RGS12 and MEK2[A];华东六省一市生物化学与分子生物学会2008年学术交流会论文摘要汇编[C];2008年
8 David Chicheong Wan;;Novel acetylcholinesterase inhibitors S-aryl-6-(4-fluorobenzyl)-7H-thiazolo[3,2-b]-1,2,4-triazin-7-one derivatives:synthesis characterization and biological activity[A];2011年全国药物化学学术会议——药物的源头创新论文摘要集[C];2011年
9 ;Fabrication and characterization of the gradient rib scaffold for tissue engineering[A];生物材料与再生医学的现状与未来——2010年第十届上海地区医用生物材料研讨会论文摘要汇编[C];2010年
10 Maurice Garcia;Thomas Fandel;Alan Shindel;Ching-Shwun Lin;Tom F.Lue;;RePairing bladcler defect by adiPose tissue-derived stem cell[A];第十六届全国泌尿外科学术会议论文集[C];2009年
中国博士学位论文全文数据库 前1条
1 王理中;牦牛驯化的基因组学证据[D];兰州大学;2016年
中国硕士学位论文全文数据库 前1条
1 李修雄;三种真菌线虫捕食性的表观遗传学基础研究和SCGB1A1基因与慢性阻塞性肺疾病的关系研究[D];云南大学;2016年
 快捷付款方式  订购知网充值卡  订购热线  帮助中心
  • 400-819-9993
  • 010-62791813
  • 010-62985026