Sensitive Bioimaging of Cellular Functional Biomolecules
【摘要】：Cellular functional biomolecules have been regarded as attractive targets for biomedical research,molecular diagnostics and disease therapy. Our recent efforts have been devoted to in situ analysis and highly selective detection of various cellular functional biomolecules and precise near-infrared cancer therapy. Here I will introduce our research results in bioimaging of cellular functional biomolecules, including electrochemical, chemiluminescent, scanometric, fluorescence, Raman and mass spectroscopic imaging for detection and in situ analysis of these molecules, such as glycans  and protein-specific glycans  on living cell surface, intracellular microRNA ,sialyltransferase and lysosomal neuraminidase , telomerase , ATP and caspases . Some nanoprobes designed for for real-time targeted imaging and precise near-infrared therapy against cancer are also discussed . The MALDI-MS quantitation of peptide and patterning of caspase activities as well as its potential for drug resistance evaluation will be reported . A recent work designs a structure switchable "lock-smart key" for cell–subtype specific siRNA delivery .Lastly, this talk will introduce a highly catalytic network by target-triggered cascade assemblyfor selective analysis and sensitive bioimaging.