Expanding puromycin based analogues capable of live cell and cell type-selective imaging
【摘要】：Global proteome analysis has provided important insights into diverse biological processes. Especially, newly synthesized proteins (NSP), a subset of the proteome, are involved in every cellular process. However, existing chemical tools used to study them have shortcomings, including limited temporal resolution and incompatibility of live-cell imaging. Herein, we first reported a suite of cell-permeable puromycin analogues capable of being metabolically incorporated into newly synthesized proteins in both live cells and neurons. Furthermore, by taking advantage of"click-to-release" reaction, we developed a versatile puromycin-based strategy for imaging and profiling of newly synthesized proteomes in a cell-specific manner.These new methods making use of puromycin overcame major drawbacks of existing strategies, thus setting the stage to directly monitor NSP from specialized samples.