Temperature effect on the dynamics of fluorescence probe for specific detection of cysteine
【摘要】：Biological thiols play pivotal roles in human physiology, such as cysteine(Cys), homocysteine(Hcy) and glutathione(GSH), design and synthesed the hight selectivity and specififity fluorescent molecular probes for selective detection of thiols of have attracted much attention. Specifically, Cys is a precursor of GSH, acetyl Co-A and taurine, as well as a source of sulfide in iron–sulfur clusters. Many human diseases are caused by abnormal levels of Cys. We have synthesized red-emitting(580 nm) molecular probe based on a styryl boron-dipyrromethene(BODIPY)/2,4-di-nitrobenzenesulfonyl(DNBS) dyad for specific detection of cysteine among the biological thiols. Adding Cys basically before and after does not have Stokes shift. The results showed that the thiol probe has excellent specificity toward cysteine over other biologically related thiols, such as glutathione. To study about the effect of different temperature on the dynamics of reactiong rate, shows that the reaction rate vary with rising temperature, and at 29℃, the reaction rate is the slowest, correspond with the types of REDOX reaction.