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《中国化学会-生物物理化学专业委员会第四届全国生物物理化学会议论文集》2016年
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Integrative Metallomic Approach to Match Metal to Proteins

孙红哲  Yau-Tsz Lai  Ligang Hu  Yuen-Yan Chang  李洪艳  Yuchuan Wang  
【摘要】:Metal ions operate as cofactors for around 40% enzymes, however, they also exhibit toxic effects. It is crucial to identify metal-protein interactions at a proteome-wide scale(1) which are difficult due to some weak and transient interactions. We developed an integrated approach consisting of gel electrophoresis and inductively coupled plasma mass spectrometry, LA-ICP-MS, IMAC, fluorescence and bioinformatic approach to identify metal-associated proteins using bismuth antiulcer drug and silver as an example(2,3). We developed a comprehensive and robust strategy to readily identify metal-associated proteins as well as to quantify the metals for fast metallome/proteome-wide profiling of metal-binding proteins. Metal-tunable fluorescence probe can track both His-tagged proteins and metal-binding proteins in live cells(4). The probe could also be used in metalloproteomics(5). We also established a bioinformatic method which allows potential metal-binding proteins both sequentially and spaciously to be searched(6,7). We show that histidine-rich proteins(HRPs) are extensively distributed in prokaryotic proteomes, with the majority of HRPs being involved in metal homeostasis. Importantly, the occurrence of histidine-rich proteins in the proteomes of prokaryotes is related to their habitats.

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