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ATP-ADP exchange activity and molecular chaperone activity of Hsp70

【摘要】:正Hsp70 is a multifunctional molecular chaperone whose interactions with protein substrates are regulated by ATP hydrolysis and ATP-ADP exchange.Hsp70 exists weakly ATPase activity and locates at the N-teminal 44-kD domain.Here,the wide-type and mutant(K71A,T204A and H227S) human Hsp70 were expressed in E.Coli and purified on HisTrap~(TM) Ni~(2+)-agarose column and then

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