Subtractive suppression hybridization combined with cDNA arrayScreening identifies differentially expressed genes between Micro- nucleates and amicronucleates of Pseudourostyla cristata

【摘要】：正 Based on morphologically comparative studies on amicronucleates and micronucleates of Pseudourostyla cristata, we supposed that the micronucleus absence may result in abnormal development of the subsurface cytoskeleton and/or other supportive elements in the vicinity of the AZM. To further provide insights into how micronucleus of P. cristata to regulate oral development, we employed suppression subtractive hybridization (SSH) and cDNA array to identify genes which express in micronucleate cells but not in amicronucleate cells.