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《国际心脏研究会(ISHR)中国分会第十届暨中国病理生理学会心血管专业委员会第十三届学术会议论文摘要文集》2008年
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Astragaloside attenuates cardiac matrix deposition in dilated cardiomyopathy associated with TGF-β1

【摘要】:正To examine whether astragaloside restrains cardiac fibrosis and investigate the underlying mechanisms in CVB3-induced murine dilated myocardiopathy (DCM).In vivo,Balb/c mice were inoculated i.p.repeatedly with CVB3 by increment to establish animal model of DCM (n=30).Other mice were given with astragaloside (Astra) (0.6 mg/kg per day) after CVB3-infection as DCM+Astra (n=20) and meanwhile control mice(n=10) were treated with same volume of EMEM without CVB3.Serum concentrations of PINP and PICP of collagen typeⅠwere detected and the PICP/PINP ratio,an index of myocardial collagen accumulation was calculated.The changes of collagen in myocardium were stained with Sirius Red and observed by polarimicroscope.CVF and collagenⅠwere measured assisted with image analysis software.The abundance of TGF-β1 was determined by Western blot. Expressions of collagen typeⅠmRNA was confirmed by reverse transcriptiona polymerase chain reaction (RT- PCR).In vitro,cultured cardiocytes were divided into four groups (A,B,C and D group).A group was control group,B group was administered with astragaloside,C group was infected with CVB3,and D group was administered with astragaloside after infected CVB3.The levels of TGF-β1 protein in four groups were detected by Western blot. The great increases of PICP,PICP/PINP,CVF and collagen typeⅠmeasured by histopathology and serology respectively in DCM mice were suppressed significantly by astragaloside.Elevated expression of TGF-β1 in DCM mice was downregulated treated with astragaloside.The mRNA abundance of collagen typeⅠin myocardium was reduced with astragaloside treatment.Compared with A group,meanwhile,the level of TGF-β1 protein was elevated in C group,and downregulated significantly in D group in vitro.These results indicate that astragaloside has antifibrotic actions in the DCM which are associated with less expression of TGF-β1 directly.
【分类号】:R259

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