收藏本站
《第六届生物技术药物理化特性分析与质量研究技术研讨会资料汇编》2018年
收藏 | 手机打开
二维码
手机客户端打开本文

Incorporating Mass Spectrometry into Biologic QC Control Environments for O-Glycan Analysis

Mark Hilliard  Ciara A.McManus  William Alley  Ying Qing Yu  John Gebler  Pauline M Rudd  
【摘要】:O-linked glycans are typically more challenging to analyze than N-linked glycans,partly because of the diversity of the core structures as well as the lack of a universal O-glycanase.Further,most O-glycan workflows are performed on high-end mass spectrometry instruments which are not available in most QC environments.These MS instruments do not provide the quantitative data required by the regulators and there can be a lack of skilled personnel to operate them.With the emergence of O-glycosylated biotherapeutics,it is now necessary to develop alternative workflows;first a more research focused LC-QTof approach and secondly,a more QC compatible methodology employing a novel mass detector,the QDa.Researchers typically employ chemical techniques for O-linked glycan release.Here we present a microwave-assisted O-linked glycan release workflow that can be completed within 12-70 minutes in comparison to traditional beta-elimination methods which takes around 16 hours.The addition of the QDa mass detector allowed for the addition of mass data that is complementary to more traditional optical detectors that are found within the QC environment.Further,we can increase MS sensitivity with the addition of an MS compatible fluorophore which also facilitates the collection of qualitative and quantitative LC-MS data which is not generally represented in current assays.For more complex samples or where accurate mass and MS/MS data is required,our LC-MS method can be transferred effortlessly to more research orientated instruments such as the QTof-MS.
【作者单位】:NIBRT,Foster Avenue,Mount Merrion,Blackrock,Co Dublin Waters Corporation
【分类号】:O657.63;O636.1

【相似文献】
中国期刊全文数据库 前10条
1 汪闻轩;梁宇学;;Introduction of A Study on Diffusion of Sewage Water in River (2)[J];中学生数学;2017年11期
2 Feng-bin Liu;Zheng-kun Hou;Yun-ying Yang;Zheng-zheng Zhang;Di Xie;Nelson Xie;Hong Thach Nguyen;;Literature review and analysis of the development of health outcomes assessment instruments in Chinese medicine[J];Journal of Integrative Medicine;2013年02期
3 Feng-bin Liu;Zheng-kun Hou;Yun-ying Yang;Pei-WU Li;Qian-wen Li;Nelson Xie;Jing-wei Li;Xiang-jing Zeng;;Literature review and analysis of the application of health outcome assessment instruments in Chinese medicine[J];Journal of Integrative Medicine;2013年03期
4 Rongjuan Li;Youxiang Sun;;Research on market-based policy instruments for eco-environmental protection[J];Chinese Journal of Population,Resources and Environment;2013年02期
5 ;Co-seismic changes of Wenchuan Ms8.0 earthquake and six aftershocks recorded by four deformation instruments at Xuzhou seismostation[J];Geodesy and Geodynamics;2011年02期
6 周强;The Friction and Wear of Sleeve-ring Pair Lubricated by Active Lubricants in the Presence of Magnetic Field[J];Journal of Wuhan University of Technology-Materials Science;2004年S1期
7 ;Distribution of China's Copper Processing Industry by Region and its Future Development[J];China Nonferrous Metals Monthly;2002年09期
8 ;Brief Regulation for Advertisement in Natural Gas Industry[J];天然气工业;1989年01期
9 ;13.Mieronaire Reading[J];纤维标准与检验;1989年06期
10 ;The Economical life of the Yao people!at Hsiang Hien and Ping-nan Hsien.[J];地理学报;1937年00期
中国重要会议论文全文数据库 前10条
1 Mark Hilliard;Ciara A.McManus;William Alley;Ying Qing Yu;John Gebler;Pauline M Rudd;;Incorporating Mass Spectrometry into Biologic QC Control Environments for O-Glycan Analysis[A];第六届生物技术药物理化特性分析与质量研究技术研讨会资料汇编[C];2018年
2 Adam Dziwoki;Kate Motyczka;Andreas Glenz;;PREVAC-a world leading manufacturer of UHV scientific research instruments and systems for the investigation of chemical and physical properties of solid state surfaces,thin films and nanomaterials[A];中国化学会第30届学术年会摘要集-第一分会:表面物理化学[C];2016年
3 ;Flexible Evaluation System for the Flexible Platform Oriented Towards the Research and Development of Modern Instruments[A];Proceedings of the 2011 Chinese Control and Decision Conference(CCDC)[C];2011年
4 Wei Lin;;High-dimensional instrumental variables estimation and inference with possibly invalid instruments[A];第十届海峡两岸统计与概率研讨会摘要集[C];2016年
5 Kurt Pawlik;;The Emerging Third Psychological Discipline:Real - life Behavior[A];第十二届全国心理学学术大会论文摘要集[C];2009年
6 Fuzhong Weng;;Advanced Radiative Transfer Modeling System and Its Applications in Satellite Data Assimilation[A];第35届中国气象学会年会 S9 卫星资料同化[C];2018年
7 ;A Study on Measurement in Quantum Control[A];’2004系统仿真技术及其应用学术交流会论文集[C];2004年
8 Chun-Keung Loong;;Neutronic consideration of pulsed-source scattering instruments[A];第三届散裂中子源多学科应用研讨会论文集[C];2006年
9 ;Smartline-the smartest way to do HPLC[A];第六届化学工程与生物技术展览暨会议参展商新产品和服务报告[C];2004年
10 Dag leff-Hallstein;;Complicated instruments revised version-CHS[A];中国医院协会第十四届全国医院感染管理学术年会资料汇编[C];2007年
中国博士学位论文全文数据库 前1条
1 王碎抗;OsMOGS参与水稻N-glycan形成和生长素介导的根系发育[D];浙江大学;2013年
中国硕士学位论文全文数据库 前10条
1 Maria Montserrat Otero de Miguel;“木头和弦”商业计划书[D];上海外国语大学;2018年
2 Clayton Elieisar(克莱顿);VR空间里的交互式3D交响乐设计及其传播效果研究[D];中国科学技术大学;2017年
3 Huang Zheng Jun;[D];复旦大学;2010年
4 韩金岩;[D];中国医科大学;2010年
5 Felipe Serrano Martinez;测试系统中用于测量和仪器控制的基于VISA的USB通信[D];东北师范大学;2012年
6 居林玲;血清糖蛋白双岩藻糖基化N-glycan的升高以及它在乳腺癌预后中的价值[D];苏州大学;2015年
7 邵盛岚;影响有稿同传质量的因素及其应对策略[D];福建师范大学;2013年
8 和运;基于液质联用的胃癌血清中O-糖组学分析[D];苏州大学;2015年
9 郭小鸿;运用既定工具在大学英语阅读中实施学生自我评价的研究[D];辽宁师范大学;2009年
10 吉佤妃(TCHEWAFEI Abide);中国与多哥金融市场工具比较[D];北京工业大学;2016年
 快捷付款方式  订购知网充值卡  订购热线  帮助中心
  • 400-819-9993
  • 010-62791813
  • 010-62985026