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《第十四届全国中药和天然药物学术研讨会论文摘要》2014年
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Study on Different Factors Affecting the Bionic Enzymatic Hydrolysis of Icariin,Epimedin A,Epimendin B,Epimendin C and Eplmedium Flavonolds

Xia Gao  Yan Chen  Jing Zhou  Cong-Yan Liu  
【摘要】:Flavonoids were the main active ingredient in Epimedium and mainly included icariin,epimedin A,epimendin B,epimendin C and baohuoside.Modern pharmacological studies have shown that Epimedium flavone had clear anti-osteoporosis effect.In order to obtain an improved absorption,transformation to secondary glycosides by intestinal enzymes and intestinal bacteria is necessary after the oral administration[1].However,intestinal enzymes and intestinal bacteria were unstable and could be affected by race,age,diet and medication[2].To screen effective and safe hydrolase in vitro and add it to epimedium flavone preparation could promote oral absorption of epimedium flavonoids,increase its bioavailability and improve its efficacy.This study aims to screen effective and safe hydrolase,investgate different factors which affect the bionic enzymatic hydrolysis of epimedium flavonoids and optimize the reaction conditions for building a novel bionic enzymolysis drug delivery system.To simulate the environment in vivo,37 °C was set as the temperature and artificial intestinal juice and gastric juice was selected as the buffer solutions.Taking the conversion of epimedium flavonoids as index,the effects of the kinds of enzyme,the ratio of enzyme and substrate,substrate concentration,reaction time,pancreatin were investigated.The results show the conversion of epimedium flavonoids was highest and their enzymolysis products was accordant with the intestinal metabolites by using snailase in artificial intestinal juice.Moreover,the main flavonoids could be completely transformed into the secondary glycoside or aglycone during 2~6 h.
【作者单位】:Jiangsu Provincial Academy of Chinese Medicine
【分类号】:R285

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