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A novel 2-keto-d-gluconic acid high-producing strain Arthrobacterglobiformis JUIM02

孙雷  
【摘要】:Objective To evaluate the 2-keto-D-gluconic acid(2 KGA) synthetic capacity of a novel 2 KGA producing strain JUTM02,which was identified as Arthrobacterglobiformis.Results The 2 KGA synthetic capacity of A.globiformis JUIM02 was evaluated by both fermentation and bioconversion,with 180 g l~(-1) dextrose monohydrate as substrates,in shake flasks and 5 1 fermenters.For fermentation,2 KGA titer,yield,molar yield and productivity of JUIM02 reached 159.05 g l~(-1),0.97 g g~(-1),90.18%and 6.63 g l~(-1) h~(-1) in 24 h.For non-sterile and buffer-free bioconversion by free resting cells(-3.2 g l~(-1) DCW) of JUFM02,these data were 172.96 g l~(-1),1.06 g g~(-1),98.07%and 5.41 g l~(-1) h~(-1) in 32 h.Moreover,JUIM02 resting cells could be repeatedly used.Resting cells stored at 4 ℃within 30 days showed stable bioconversion capacity,with 2 KGA titers 171.50 g l~(-1),yields 1.04 g g~(-1) and molar yields 97.24%.ConclusionA.globiformis JUIM02 is a promising 2 KGA industrial-producing strain suitable for various production methods.

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