【摘要】：正BACKGROUND:Bone morphogenetic protein-15(BMP-15),an oocyte-derived growth factor,has been proved to be critical for normal fertility in female mammals.However,no dependence relation has been established between BMP-15 and oocyte quality in human ovary.The aim of this study was to investigate the BMP-15 levels in human follicular fluid and its role in assessing oocyte quality and developmental potential. METHODS:A total of 82 infertile patients undergoing the first in vitro fertilization(VIF)because of tubal obstruction participated in this study.The oocytes of each patient's first aspirated follicle were individually matured in vitro,and the follicular fluid of these follicles was nanalyzed for BMP-15,FSH, 17β-estradiol(E2)and progesterone levels by western blot and radioimmunoassay,respectively. Fertilization,cleavage rate and embryo quality outcomes and steroid hormones in FF were related to follicular fluid concentration ofBMP-15. RESULT:The clinical characteristics of patients from the two groups were similar in terms of age,body mass index,base FSH,induction days,FSH units used,number of retrieved oocytes,and fertilization rate. A significant correlation was found between the BMP-15 levels and oocytes cleavage and embryo quality. BMP-15 levels in FF were significantly correlated with the E2 concentrations,but not FSH and progesterone. CONCLUSIONS:Our study demonstrates that BMP-15 level is a reliable assessment ofoocytes quality, especially for cytoplasmic quality.