Production of recombinant enveloped structural proteins from the Chinese WSSV isolate

【摘要】：正The white spot syndrome virus (WSSV) is one of the deadly pathogens of penaeid shrimps and other crustaceans. The WSSV virion consists of an enveloped rod-shaped nucleocapsid enclosing a large circular double stranded DNA genome of 305 kb with 181 open reading frames. The two major structural genes, VP19 and VP28 were amplified from the genomic DNA of Chinese isolate of WSSV and cloned in pUCm-T vector and sub cloned in pET-30a (+) vector. The expressions of genes in E. coll (BL21) were confirmed by SDS-PAGE analysis. The clones were sequenced, submitted to the gene bank and the WSSV (Xiang Shan strain) was compared with the previous reported sequences of WSSV of various regions, and was revealed that VP19 and VP28 gene sequences had certain differences from the sequences of similar genes of the isolates have already been reported. The recombinant proteins expressed, purified and characterized. The developed antibodies against VP19 and VP28 showed very good titer with the serum of WSSV infected shrimps and not with the serum from uninfected shrimps.