收藏本站
收藏 | 手机打开
二维码
手机客户端打开本文

Production of recombinant enveloped structural proteins from the Chinese WSSV isolate

Rajeev Kumar Jha  
【摘要】:正The white spot syndrome virus (WSSV) is one of the deadly pathogens of penaeid shrimps and other crustaceans. The WSSV virion consists of an enveloped rod-shaped nucleocapsid enclosing a large circular double stranded DNA genome of 305 kb with 181 open reading frames. The two major structural genes, VP19 and VP28 were amplified from the genomic DNA of Chinese isolate of WSSV and cloned in pUCm-T vector and sub cloned in pET-30a (+) vector. The expressions of genes in E. coll (BL21) were confirmed by SDS-PAGE analysis. The clones were sequenced, submitted to the gene bank and the WSSV (Xiang Shan strain) was compared with the previous reported sequences of WSSV of various regions, and was revealed that VP19 and VP28 gene sequences had certain differences from the sequences of similar genes of the isolates have already been reported. The recombinant proteins expressed, purified and characterized. The developed antibodies against VP19 and VP28 showed very good titer with the serum of WSSV infected shrimps and not with the serum from uninfected shrimps.

知网文化
【相似文献】
中国重要会议论文全文数据库 前1条
1 Rajeev Kumar Jha;;Production of recombinant enveloped structural proteins from the Chinese WSSV isolate[A];生物技术对水产业的促进作用——2005水产科技论坛论文集[C];2005年
中国知网广告投放
 快捷付款方式  订购知网充值卡  订购热线  帮助中心
  • 400-819-9993
  • 010-62982499
  • 010-62783978