收藏本站
《中国生理学会生殖科学专业委员会—中国动物学会生殖生物学分会第二次联合学术年会暨“生殖科学专业委员会第二次学术交流会”和“生殖生物学分会第十六次学术年会”论文集》2017年
收藏 | 手机打开
二维码
手机客户端打开本文

Effects of Prokineticin 2 on Testicular Microenvironment in Rats

Ying Li  Zhiyong Hu  Kai Zhao  Huiping Zhang  
【摘要】:Prokineticin2(PK2),a pro-inflammatory peptide,was expressed in primary spermatocytes of testes.This study aimed to investigate the effects of PK2 on testicular microenvironment in rats.Immunized with testicular homogenate was applied to establish rats experimental autoimmune orchitis(EAO) model The expression of PK2 and its receptor(prokineticin receptor 1,PKRl) were detected in orchitis at 50 and 80 days after the first immunization.HEK293 cells were co-transfected by PK2-siRNA plasmid and PK2 over-expression plasmid containing a ProLabel-tag.The most efficient siRNA sequence was injected to the rete testes of 12-day-old juvenile male SD rats to down-regulate the expression of PK2 in vivo.In addition,PK2 was over-expressed in rats testes by injecting with PK2 protein through the testes rete of adult SD rats in different concentrations(50 pmol,100 pmol and 300 pmol).Testicular morphology of low-expression or over-expression of prokineticin2 in rats testes was detected by staining with HE.Immunohistochemistry was used to detect the expression of inducible nitric oxide synthase that associated with the oxidative stress reaction.The results showed that up-expression PK2 and PKRl in EAO-induced models at 50 days after the first immunization.Over-expression of PK2 contributed to the apoptosis of spermatogenic epithelial cells and the increased infiltration of the inflammatory cells,while that of low-expression of PK2 had no change.Furthermore,the expression of inducible nitric oxide synthase was increased significantly when PK2 was over-expressed.This data demonstrated that the PK2 may have negative influence on testicular microenvironment through inducible nitric oxide synthase in rats.Interference of PK2 may represent a novel and promising therapeutic strategy for the clinical management of orchitis.

【相似文献】
中国期刊全文数据库 前10条
1 冯艳,刘钊,曹慧青,孟宪敏,瞿智玲,熊密,邓仲端;Construction of Eukaryotic Expression Plasmid of Human PRX3 and Its Expression in HEK-293FT Cells[J];华中科技大学学报(医学英德文版);2004年04期
2 ;Construction of plasmid containing human blood- clotting factor Ⅷ and observation its expression in Cos-7 cells[J];实验血液学杂志;1997年03期
3 周建华,Roberto Petracca;Influence of Single Base Change in Shine-Dalgarno Sequence on the Stability of B.Subtilis Plasmid PSM604[J];Journal of Tongji Medical University;2000年03期
4 ;Construction and Expression of Eukaryotic Expression Vector and Plasmid Expressing siRNA of Human Protection of Telomeres 1[J];生物医学工程学杂志;2005年S1期
5 温肇荣;;The Construction fo Hybrid Plasmid pBR322:RSF1010[J];Journal of Medical Colleges of PLA;1986年01期
6 翁亚丽,刘雁雁,贾辅忠,梅亚宁,赵旺胜;Analysis of Drug Resistance Spectra and Conjugative Plasmid Carrying Rates of P.aeruginosa and Acinetobacter[J];JOURNAL OF NANJING MEDICAL UNIVERSITY;1997年01期
7 汤屹,刘文励,周剑锋,徐慧珍,路武;Transfection Efficiency Comparison of Oligonucleotide and Plasmid to the HL-60 Cell Line with Liposomes[J];华中科技大学学报(医学英德文版);2005年01期
8 Yingzi Lin;Shixiang Bao;Guanghong Tan;Fengying Huang;Huiqin Huang;;Construction and identification of recombinant plasmid pUIS3-BLC~+[J];Asian Pacific Journal of Tropical Medicine;2010年10期
9 胡燕华,黄琼,姜发纲,陈宏;The Expression of the Plasmid DNA Encoding TGF-β_1 in Endothelium after Injection into the Anterior Chamber[J];华中科技大学学报(医学英德文版);2002年04期
10 Shin-ichiMoriwaki,TakashiYagi,ChikakoNishigori,HirakuTakebe;ANALYSIS OF N-METHYL-N-NITROSOUREA-INDUCED MUTATIONS IN A SHUTTLE VECTOR PLASMID PROPAGATED IN MOUSE O~6-METHYLGUANINE-DNA METHYLTRANSFERASE-DEFICIENT CELLS IN COMPARISON WITH PROFICIENT CELLS.[J];癌变.畸变.突变;1991年S1期
中国重要会议论文全文数据库 前10条
1 ;Plasmid cDNA Libraries to NIH3T3, Cloning of Novel Tumor related Genes from the Cell Line Anip-973[A];第六次全国医学遗传学学术会议文摘汇编[C];2005年
2 向华;孙超岷;厉云;周梅先;梅双双;陆秋鹤;周利刚;;Access to the fundamental of haloarchaeal genetics:from plasmid replication and halocin production[A];第二届中国青年学者微生物遗传学学术研讨会论文集[C];2006年
3 ;The conserved CRISPR and interacting Cas proteins in Streptomyces linear plasmid pSHK1 and in other actinomycetes[A];2008年中国微生物学会学术年会论文摘要集[C];2008年
4 ;Characterization of replication and conjugation of plasmid p WTY27 from a widely distributed Streptomyces species[A];2012年第五届全国微生物遗传学学术研讨会论文摘要集[C];2012年
5 ;Optimization of Electroporation Processing of Bacillus subtilis[A];Proceedings of Conference on Environmental Pollution and Public Health(CEPPH 2012)[C];2012年
6 ;Investigations of the osteogenic potential of the hBMP2 gene activated nanobone putty[A];2007年贵州省医学会骨科学分会学术年会论文汇编[C];2007年
7 ;In vitro pharmacokinetics and pharmacodynamics of Ievofloxacin against plasmid-mediated quinolone resistant Escherichia coli[A];中国药理学会临床药理学专业委员会会议暨第十次全国临床药理学学术会议论文集[C];2007年
8 Yongchao Wang;Hong Liu;Jiahe Peng;Liangpeng Li;Yan Zhang;Jinyu Dong;Xiaohua Liu;Yu Jiang;;Farnesoid X receptor up-regulates expression fibroblast growth factor 21 in HepG2 and the study of its mechanism[A];第11届全国脂质与脂蛋白学术会议论文汇编[C];2012年
9 周水洪;;Construction and Identification of an Antisense Glucose Transporter-1 Plasmid[A];华东六省一市耳鼻咽喉-头颈外科学术会议暨2008年浙江省耳鼻咽喉-头颈外科学术年会论文汇编[C];2008年
10 ;Establishment of a real-time PCR assay for detection of Giardia from Dogs[A];中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十一次学术研讨会论文集[C];2011年
中国博士学位论文全文数据库 前1条
1 TRAN VU HAI(陈武海);γ-TMT和FAD2基因在大肠杆菌与里氏木霉中的表达及鉴定[D];华东理工大学;2013年
中国硕士学位论文全文数据库 前2条
1 Fransiska Yuanita Wijaya(黄诗佳);[D];重庆医科大学;2011年
2 杨艳丽;[D];陕西师范大学;2011年
 快捷付款方式  订购知网充值卡  订购热线  帮助中心
  • 400-819-9993
  • 010-62791813
  • 010-62985026