收藏本站
《中国生理学会内分泌代谢、比较生理与应激生理学术会议论文摘要汇编》2017年
收藏 | 手机打开
二维码
手机客户端打开本文

Diet-induced reconstruction of mucosal microbiota associated with alteration of epithelium lectin expression and regulation in maintenance of rumen homeostasis

Hong Shen  Zhihui Xu  Zanming Shen  Zhongyan Lu  
【摘要】:Objective The mucosal microbiota in the gastrointestinal(GI) tract plays a pivotal role in local and systemic host physiology.Lectins are evolutionally conserved pattern-recognition receptors(PRPs) mediating the agglutination and immobilization of recognized pathogens.Whether lectins of epithelial cells(ECs) contribute to the recognition of mucosal microbes and the activation of tolerogenic cytokines in the gastrointestinal(GI) tract of animals is unknown.Methods We applied an integrated method of RNA-seq and 16 S rRNA gene sequencing to investigate the alteration of epithelial lectin expression and regulation with a diet-induced reconstruction of mucosal microbiota in goat rumen.Results Our results showed that the diversity and richness of rumen mucosal microbiota were promoted by the low energy diet.Meantime,in rumen epithelium,five lectin genes,namely sialic acid-binding Ig-like lectin 14(LOC102180073),C-type lectin domain family 4,member E(CLEC4 E),C-type lectin domain family 7,member A(CLEC7 A),C-type lectin domain family 16,member A(CLEC16 A),and lectin,mannose-binding 2(LMAN2) were indicated to promote the expressions of 8 cytokine genes,transforming growth factor beta 1(TGFB1),and 4 enzyme genes involved in retinoic acid biosynthesis via 6 signaling pathways.The analysis of combined data showed 8 microbial genera(Clostridium_IV,Desulfobulbus,Eubacterium,Ochrobactrum,Propionibacterium,Pseudomonas,Slackia,Staphylococcus and Subdivision5_genera_IS) were highly related to the expression of functioned lectins.These findings provide new insights into the interaction between the epithelium and mucosal microbiota in the maintenance of GI homeostasis.
【作者单位】:College of Life Science,Nanjing Agricultural University Bioinformatics Center,Nanjing Agricultural University Key Lab of Animal Physiology and Biochemistry,College of Veterinary Medicine,Nanjing Agricultural University
【分类号】:S852.6
【正文快照】:
Diet-induced reconstruction of mucosal microbiota associated with alteration of epithelium lectin expression and regulation in maintenance of rumen homeostasis@Hong Shen$College of Life Science,Nanjing Agricultural University!Nanjing,Jiangsu,China$Bioinf

中国知网广告投放
 快捷付款方式  订购知网充值卡  订购热线  帮助中心
  • 400-819-9993
  • 010-62791813
  • 010-62985026