收藏 | 手机打开

An EPSPS+GAT transgenic glyphosate-resistant soybean line impact on the diversity of root-associated bacterial community

Min-Kai Yang  Zhong-Ling Wen  Yun-Ting Lu  Xin-Hong Xu  Jing Cheng  Zhi Hong  Jin-Liang Qi  Gui-Hua Lu  Yong-Hua Yang  
【摘要】:At present, genetically modified crops have been widely planted in the world, with obvious economic benefits. However,a series of biosafety problems have also arisen. In the past, researchers paid more attention to the biosafety of the above ground part of the crops, while the research on the underground part is less. In this study, the soybean root-associated bacterial communities of 5-enolpyruvylshikimate-3-phosphate synthase(EPSPS) plus glyphosate N-acetyltransferase(GAT) transgenic soybean line Zhonghuang 10-6(Z106), its recipient variety Zhonghuang 10(ZH10), and Zhonghuang 10-6 treated with glyphosate(Z106 J) were compared at the seedling, flowering, and pod-setting stages by high-throughput sequencing of V4 hypervariable regions of 16 S rRNA gene(16 S rDNA) amplicons via Illumina MiSeq. The results of analysis of 16 S rDNA amplicons high-throughput sequencing data showed no significant difference in alpha/beta diversity of root-associated bacterial communities at above three stages among ZH10, Z106, and Z106 J under the field growth conditions. Afterwards,we made ternary phase diagram to further analyze the significant difference among the three treatments. The ternary phase diagram result demonstrated the relative abundances of the species. The results showed that the Ensifer_fredii was significantly higher in ZH10 and Z106 J than in Z106.Ensifer_fredii is a rhizobial species exhibiting a extremely broad nodulation host-range and belong to nitrogen-fixing bacterial genera.The further analysis revealed that the relative abundance of Ensifer_fredii was higher in root samples at flowering and pod-setting stages. Additionally, significant differences among ZH10, Z106, and Z106 J were detected by the relative abundances of main nitrogen-fixing bacterial genera at seedling, flowering, and pod-setting stages. In conclusion,There were significant differences in the effects of transgenic and recipient soybeans on soil microbial communities, especially on the relative abundance of nitrogen fixing microbial communities.

中国期刊全文数据库 前20条
1 Wenting Li;Yujun Quan;Mengmeng Zhang;Kejun Wang;Muzhen Zhu;Ye Chen;Qiuyan Li;Keliang Wu;;Effects of pituitary-specific overexpression of FSHα/β on reproductive traits in transgenic boars[J];Journal of Animal Science and Biotechnology;2018年01期
2 ;Enhanced hyperplasia in muscles of transgenic zebrafish expressing Follistatin1[J];Science China(Life Sciences);2011年02期
3 ;Field evaluation of effects of transgenic cry1Ab/cry1Ac,cry1C and cry2A rice on Cnaphalocrocis medinalis and its arthropod predators[J];Science China(Life Sciences);2011年11期
4 ;Integration mechanisms of transgenes and population fitness of GH transgenic fish[J];Science China(Life Sciences);2010年04期
5 ;Generation of fad2 transgenic mice that produce omega-6 fatty acids[J];Science in China(Series C:Life Sciences);2009年11期
6 ;Progress in the evaluation of transgenic fish for possible ecological risk and its containment strategies[J];Science in China(Series C:Life Sciences);2007年05期
7 ;Generation and characterization of a transgenic mouse model for pancreatic cancer[J];World Journal of Gastroenterology;2006年17期
8 Elizabeth D. Earle;Anthony M. Shelton;;Insect Resistance Management Assessment of Transgenic Plants Expressing Two Bt Toxins[J];Journal of Shanghai Jiaotong University;2005年S1期
9 ;Differentiation of bone mar-row derived Thy-1~+β_2M~- cells into hepatocytes induced by coculture with transgenic CFSCs[J];Chinese Science Bulletin;2004年09期
10 ;An expression profile of human α-lactalbumin in the milk of transgenic mouse[J];Science in China(Series C:Life Sciences);2004年03期
11 ;CAS Researcher Awarded for Transgenic Technologies[J];Bulletin of the Chinese Academy of Sciences;2004年03期
12 焦德茂,匡廷云,李霞,戈巧英,黄雪清,郝乃斌,白克智;Physiological characteristics of the primitive CO_2 concentrating mechanism in PEPC transgenic rice[J];Science in China(Series C:Life Sciences);2003年04期
13 ;Impact Evaluation of Insect-Resistant Transgenic Rice on the Feeding and Oviposition Behavior of Its Non-Target Insect, the Brown Planthopper, Nilaparvata lugens (Homptera: Delphacidae)[J];Agricultural Sciences in China;2003年09期
14 ;Field Evaluation of the Asian Corn Borer Control in Hybrid of Transgenic Maize Event MON 810[J];Agricultural Sciences in China;2003年12期
15 ;Oral immunization of animals with transgenic cherry tomatillo expressing HBsAg[J];World Journal of Gastroenterology;2003年05期
16 孙汉堂,肖明振,吴补领,费俭;Construction of transgenic vector of mouse dentin sialoprotein[J];中国临床康复;2002年23期
17 ;Expression activity of the Cp-TI gene in transgenic rice plants[J];Chinese Rice Research Newsletter;2001年03期
18 李旭刚;Increase of Expression Levels of Reporter Gene in Transgenic Tobaccos by Matrix Attachment Regions[J];High Technology Letters;2000年04期
19 胡以平;邱信芳;薛京伦;刘祖洞;;Regulation of human clotting factor IX cDNA expression in transgenic mice[J];Science in China(Series B);1995年07期
中国重要会议论文全文数据库 前10条
1 Zhong-Ling Wen;Yun-Ting Lu;Min-Kai Yang;Gu-Hao Wang;Xiao-Mei Hua;Jin-Liang Qi;Gui-Hua Lu;Yong-Hua Yang;;Enrichments/Derichments of root-associated bacteria related to nitrogen and phosphorus cycling caused by the growth of an EPSPS-transgenic maize line in the field[A];2018中国遗传学会第十次全国会员代表大会暨学术讨论会论文摘要汇编[C];2018年
2 Min-Kai Yang;Zhong-Ling Wen;Yun-Ting Lu;Xin-Hong Xu;Jing Cheng;Zhi Hong;Jin-Liang Qi;Gui-Hua Lu;Yong-Hua Yang;;An EPSPS+GAT transgenic glyphosate-resistant soybean line impact on the diversity of root-associated bacterial community[A];江苏省遗传学会2019年学术研讨会论文集[C];2019年
3 陆桂华;Cheng-Yi Tang;Xiao-Mei Hua;Jing Cheng;Gu-Hao Wang;Yin-Ling Zhu;Li-Ya Zhang;Hui-Xia Shou;Jin-Liang Qi;杨永华;;Effects of an EPSPS-transgenic soybean line ZUTS31 on root-associated bacterial communities during the Field Growth[A];江苏省遗传学会2017年学术研讨会——“技术创新与遗传学发展”论文摘要集[C];2017年
4 Hong-Yu Wu;Qing-Qing Yang;Qiao-Quan Liu;;Biofortification of rice with the essential amino acid lysine[A];江苏省遗传学会2017年学术研讨会——“技术创新与遗传学发展”论文摘要集[C];2017年
5 Lin Sun;Chao Lin;Yunzhi Song;Jinwen Du;Fujiang Wen;Changxiang Zhu;;Dimeric artificial microRNAs mediate highly efficient RSV and RBSDV resistance in transgenic rice plants[A];植物病理学研究进展——中国植物病理学会第十二届青年学术研讨会论文选编[C];2015年
6 李圆;;Generation and characterization of MMTV-MUC1-CD transgenic mouse model[A];中国遗传学会模式生物与人类健康研讨会会议论文集[C];2010年
7 ;Generation of a fluorescent transgenic zebrafish for detection of environmental estrogens[A];2010·第一届斑马鱼学术研讨会论文摘要集[C];2010年
8 Cook DR;;Large scale isolation of organ specific expression genes and characterization of pod specific promoters for transgenic application in peanut(Arachis hypogaea L.)[A];第十三届全国植物基因组学大会论文集[C];2012年
9 Jinling Kang;Anna Snapp;Ruojin Wang;Changlu Wang;Chaofu Lu;;Production of hydroxylated fatty acids in transgenic seeds of camelina[A];第十四届全国植物基因组学大会摘要[C];2013年
10 Zhuona Rong;Tingting Fan;Huiling Li;Juan Li;Fujin Wang;Jingyu Wang;Aiguo Wang;;Proteomic analysis of hepatic tumor in Hras12V transgenic mice[A];2017年第十五届中国北方实验动物科技年会论文集[C];2017年
中国博士学位论文全文数据库 前10条
1 Shaukat Ali;[D];浙江大学;2005年
2 AMANI MANNAKKARA;[D];华中农业大学;2013年
3 Bello Babatunde Kazeem;棉花蔗糖非酵解型蛋白激酶GhSnRK2的克隆及功能分析[D];中国农业科学院;2014年
4 Afsana Ansari;利用RNAi技术创制水稻矮秆不育材料的研究[D];中国农业科学院;2016年
5 鞠辉明;生长激素可控表达转基因猪的制备[D];中国农业科学院;2012年
6 吴蕾;[D];内蒙古大学;2011年
7 吴伟祥;转Bt基因克螟稻对土壤和根系微生物多样性的影响[D];浙江大学;2003年
8 Hussain Javed Umrani;ABP9过表达转基因玉米在大田中的抗旱表现[D];中国农业科学院;2016年
9 EHSAN ULLAH KHAN;[D];华中农业大学;2010年
10 马馨;转赖氨酸基因小鼠和牛的研究[D];吉林大学;2012年
中国硕士学位论文全文数据库 前2条
1 Kashif Rafiq Zahid;[D];华中农业大学;2014年
2 巴沙尔(BACHAR DAHRO);枳碱性/中性转化酶基因PtrA/NINV功能分析[D];华中农业大学;2016年
 快捷付款方式  订购知网充值卡  订购热线  帮助中心
  • 400-819-9993
  • 010-62982499
  • 010-62783978