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《江苏省遗传学会2019年学术研讨会论文集》2019年
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An EPSPS+GAT transgenic glyphosate-resistant soybean line impact on the diversity of root-associated bacterial community

Min-Kai Yang  Zhong-Ling Wen  Yun-Ting Lu  Xin-Hong Xu  Jing Cheng  Zhi Hong  Jin-Liang Qi  Gui-Hua Lu  Yong-Hua Yang  
【摘要】:At present, genetically modified crops have been widely planted in the world, with obvious economic benefits. However,a series of biosafety problems have also arisen. In the past, researchers paid more attention to the biosafety of the above ground part of the crops, while the research on the underground part is less. In this study, the soybean root-associated bacterial communities of 5-enolpyruvylshikimate-3-phosphate synthase(EPSPS) plus glyphosate N-acetyltransferase(GAT) transgenic soybean line Zhonghuang 10-6(Z106), its recipient variety Zhonghuang 10(ZH10), and Zhonghuang 10-6 treated with glyphosate(Z106 J) were compared at the seedling, flowering, and pod-setting stages by high-throughput sequencing of V4 hypervariable regions of 16 S rRNA gene(16 S rDNA) amplicons via Illumina MiSeq. The results of analysis of 16 S rDNA amplicons high-throughput sequencing data showed no significant difference in alpha/beta diversity of root-associated bacterial communities at above three stages among ZH10, Z106, and Z106 J under the field growth conditions. Afterwards,we made ternary phase diagram to further analyze the significant difference among the three treatments. The ternary phase diagram result demonstrated the relative abundances of the species. The results showed that the Ensifer_fredii was significantly higher in ZH10 and Z106 J than in Z106.Ensifer_fredii is a rhizobial species exhibiting a extremely broad nodulation host-range and belong to nitrogen-fixing bacterial genera.The further analysis revealed that the relative abundance of Ensifer_fredii was higher in root samples at flowering and pod-setting stages. Additionally, significant differences among ZH10, Z106, and Z106 J were detected by the relative abundances of main nitrogen-fixing bacterial genera at seedling, flowering, and pod-setting stages. In conclusion,There were significant differences in the effects of transgenic and recipient soybeans on soil microbial communities, especially on the relative abundance of nitrogen fixing microbial communities.

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