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《江苏省遗传学会2019年学术研讨会论文集》2019年
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Differential impacts on the structure of rhizosphere bacterial communities between Al-tolerant and Al-sensitive soybean genotypes in acidic soil

Zhong-Ling Wen  Min-Kai Yang  Aliya Fazal  Dong-Qing Hu  Gui-Hua Lu  Jin-Liang Qi  Yong-Hua Yang  
【摘要】:Acidic soil is one of the major limiting factors for crop productivity in the world. In this study, two genotypes of soybean, aluminum-tolerant soybean BX10 and aluminum-sensitive soybean BD2 were used as plant materials and the acidic red soil was used as growth media. Soybeans were planted in rhizobox and soil samples from different sampling compartments were collected at flowering stage. According to the distance from the root, the soil layers from the inside to the outside are WRH, BRH and SRH, respectively. The rhizosphere bacterial communities were analyzed by high-throughput sequencing of V4 hypervariable regions of 16 S rRNA gene(16 S rDNA) amplicons via Illumina MiSeq. The results of alpha diversity showed that the BRH/SRH of BX10 was significantly lower on community richness than that of BD2, while the WRH existed no significant difference between BX10 and BD2. Among the three sampling compartments of the same soybean genotype(BX10 or BD2), WRH had the lowest community richness and diversity while existed the highest coverage. In the comparison of beta diversity, no significant difference of the overall rhizosphere bacterial communities existed for any compartment between the two genotypes. However, the relative abundance of major bacterial taxa at phylum level showed that the SRH of BX10 enriched Proteobacteria(especially in the genus Burkholderia), and depleted Chloroflexi(genus Ktedonobacter), as compared with BD2. On the contrary, the BRH of BX10 enriched Chloroflexi and depleted Proteobacteria than that of BD2. These bacterial genera including Burkholderia and Ktedonobacter were reported to be associated with nitrogen-fixation, and Burkholderia was also proved to be containing bacterial species with aluminum-tolerance. Interestingly, the BRH also additionally enriched Actinobacteria(genus Acidothermus) and depleted Bacteroidetes(genus Mucilaginibacter) when BX10 vs. BD2 was used. No significant difference of the relative abundance of major bacterial taxa was found at phylum level of WRH between BX10 and BD2. Furthermore, the ANOSIM and Adonis results indicated that there was also no significant difference in beta diversity among the three different sampling compartments for any same soybean genotype. The PCA/PCoA analyses showed the high similarity of species composition between the two compartments BRH and SRH of the same genotype(BX10 or BD2). Strikingly, as compared with BRH/SRH, the WRH across the same genotype(BX10 or BD2) always had an enrichment effect on rhizosphere bacteria associated with nitrogen-fixation, especially in order Bacillales,Pseudomonadales and Micrococcales; in family Bacillacea, Paenibacillacea, Pseudomonadaceae and Micrococcaceae; in genus Bacillu, Paenibacillus, Pseudomonas and Pseudarthrobacter, and in species Paenibacillus odorifer, Paenibacillus castaneae, Paenibacillus pectinilyticus, Pseudomonas brassicacearum and Pseudarthrobacter oxydans. Our results implicate that the genotype of soybean will dramatically affect the rhizosphere microbial community, especially on the bacteria related to nitrogen-fixation and/or aluminum-tolerance in the compartments of the BRH and/or SRH, but not in the WRH. However, the WRH significantly enrich nitrogen-fixing bacteria among the three compartments across the two genotypes.

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