收藏本站
《2018中国遗传学会第十次全国会员代表大会暨学术讨论会论文摘要汇编》2018年
收藏 | 手机打开
二维码
手机客户端打开本文

Study on bone formation in Ano5-knockout mice

Xiaoyu Wang  Xiu Liu  Yi Liu  Ying Hu  
【摘要】:Gnathodiaphyseal dysplasia(GDD; OMIM#166260) is a rare skeletal disorder with autosomal dominant inherit pattern. It is characterized by lesions of jawbones, thickening cortical diaphysis of tubular bones and frequent fractures as a results of minimal injury. We found previously that the silence of Ano5 gene resulted in increased mineral nodule formation in differentiating MC3 T3-E1 osteoblast precursors in vitro and findings suggests that ANO5 plays a role in osteoblast differentiation. However, the pathological effects of ANO5 deficiency on GDD in vivo has not been elucidated completely. Now we generated a Ano5-konckout mouse modal with CRISPR/Cas 9 method. The expression of Ano5 in bone tissue decreased significantly and some clinical features of human GDD have been replicated. Meanwhile, the mouse calvarial osteoblast(mCOBs) cultures were performed and the expression of osteoblast-related genes as well as bone matrix formation assays were investigated by quantitative PCR and alizarin red staining respectively. The results showed that Osteocalcin, Col1 a1, Runx2, Osterix, Osteopontin and Rankl highly elevated and mineralization enhanced drastically in Ano5-/-mCOB. The data are consistent with the achievements we observed before in vitro. We believe this new mouse model can contribute to research into the pathogenesis of skeletal abnormalities in GDD and provide the clues to develop the therapeutic approaches for GDD.

免费申请
【相似文献】
中国期刊全文数据库 前10条
1 曹小俊;;Effect of metformin on proliferation and related genes expression of human osteoblast MG63 under high glucose[J];China Medical Abstracts(Internal Medicine);2013年01期
2 Caixia Yu;Jiao Gong;Fu-ling Yin;Jian Huang;Tian-lan Zhang;Kui Wang;;Influences of LaCl_3 on the mineral phase transformation during osteoblast mineralization in vitro[J];Journal of Environmental Sciences;2017年01期
3 ;Increased osteoblast adhesion on nanophase Ti6Al4V[J];Chinese Science Bulletin;2008年11期
4 Tao Qiu;Janet L.Crane;Liang Xie;Lingling Xian;Hui Xie;Xu Cao;;IGF-I induced phosphorylation of PTH receptor enhances osteoblast to osteocyte transition[J];Bone Research;2018年01期
5 M.Noelle Knight;Kannan Karuppaiah;Michele Lowe;Sarthak Mohanty;Robert L.Zondervan;Sheila Bell;Jaimo Ahn;Kurt D.Hankenson;;R-spondin-2 is a Wnt agonist that regulates osteoblast activity and bone mass[J];Bone Research;2018年03期
6 WANG Jin-tao;YIN Xi-ya;GAO Shan;ZHOU Lu;;Overexpression of SIRT6 prevents hypoxia-induced apoptosis in osteoblast cells[J];中国药理学与毒理学杂志;2017年05期
7 Zhen-Zhen Shang;Xin Li;Hui-Qiang Sun;Guo-Ning Xiao;Cun-Wei Wang;Qi Gong;;Differentially expressed genes and signalling pathways are involved in mouse osteoblast-like MC3T3-E1 cells exposed to 17-β estradiol[J];International Journal of Oral Science;2014年03期
8 Li-yuan LI;Pan WANG;Zhao WANG;;PFMG1 promotes osteoblast differentiation and prevents osteoporotic bone loss[J];中国药理学与毒理学杂志;2015年S1期
9 Abdullah Amira Raudhah;Hapidin Hermizi;Abdullah Hasmah;;Combination treatment of bisphosphonate(pamidronate) and Quercus infectoria semipurified fraction promotes proliferation and differentiation of osteoblast cell via expression of Osterix and Runx2 marker[J];Asian Pacific Journal of Tropical Biomedicine;2018年05期
10 Jun Sun;Joerg Ermann;Ningning Niu;Guang Yan;Yang Yang;Yujiang Shi;Weiguo Zou;;Histone demethylase LSD1 regulates bone mass by controlling WNT7B and BMP2 signaling in osteoblasts[J];Bone Research;2018年03期
中国重要会议论文全文数据库 前10条
1 Xiaoyu Wang;Xiu Liu;Yi Liu;Ying Hu;;Study on bone formation in Ano5-knockout mice[A];2018中国遗传学会第十次全国会员代表大会暨学术讨论会论文摘要汇编[C];2018年
2 Wang Ling;;17-β-estradiol up-regulates apolipoprotein genes expression during osteoblast differentiation in vitro[A];第十一届全国免疫学学术大会摘要汇编[C];2016年
3 Man-ping Lin;Yan-feng Luo;Yan Li;Juan Xing;Yuan-liang Wang;;Role of surface chemistries in Fibronectin adsorption and osteoblast responses[A];第十届全国生物力学学术会议暨第十二届全国生物流变学学术会议论文摘要汇编[C];2012年
4 Jianlei Lu;Bing Yao;Yuexin Xu;Yucui Jin;Kaikai Shi;Yifang Shui;Shiyang Pan;Li Chen;Changyan Ma;;Acetylation of Osx enhances its protein stability,DNA binding ability and transcriptional activity and is required for osteoblast differentiation[A];第十四次全国医学遗传学学术会议论文汇编[C];2015年
5 Yue Tao;Minhao Wu;Krishna M Sinha;Xing Zhou;Bin Hu;Jianye Zang;;Structural Basis for Tetramerization-dependent Gene Repression by Histone Demethylase NO66[A];第四届中国结构生物学学术讨论会论文摘要集[C];2013年
6 Junqing Sun;Liang Shi;Jianbao Zhang;;Fluid shear stress induced Ca~(2+)transient in osteoblast:a model study[A];第十一届全国生物力学学术会议暨第十三届全国生物流变学学术会议会议论文摘要汇编[C];2015年
7 郭风劲;Zhangyuan Xiong;Xiaofeng Han;Fei Xia;Meiling Li;Zhimeng Wu;Li Deng;;The role of IRE1a-XBP1S in endochondral bone growth and osteoblast Differentiation[A];第三届“模式生物与人类健康”发育遗传学全国学术研讨会论文集[C];2014年
8 ;Purification and Biological Activity of Human Bone Morphogenetic Protein-7 expressed in Escherichia coli[A];华东六省一市生物化学与分子生物学会2009年学术交流会论文摘要汇编[C];2009年
9 ;Activation of PKA and phosphorylation of sodium dependent vitamin C transporter 2 by prostaglandin E2 promote osteoblast-like differentiation in MC3T3-E1 cells[A];浙江省生理科学会2008年学术年会论文汇编[C];2008年
10 ;Quantitative proteomic analysis of KEGG signaling pathways in bone morphogenetic protein-2-induced osteoblast differentiation in MC3T3-E1 cells using SILAC[A];中华医学会第六次全国骨质疏松和骨矿盐疾病学术会议暨中华医学会骨质疏松和骨矿盐疾病分会成立十周年论文汇编[C];2011年
 快捷付款方式  订购知网充值卡  订购热线  帮助中心
  • 400-819-9993
  • 010-62791813
  • 010-62985026