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The Responses of Soil Microbial Community to Abiotic Stress Imposed by Excess Copper

【摘要】:正Microorganisms are important components in soil due to their critical roles in the maintenance of soil function through their involvement in such key processes as soil structure formation,organic matter decomposition,toxin removal,as well as the cycling of carbon,nitrogen and phosphorus.They also show sensitivity to the change of soil microcosm such as the contamination of chemicals.Copper-based fungicides are widely applied in agriculture for the control of fungal and bacterial diseases.Copper ion in soil is toxic to the microbes.However,it is seldom known whether the toxicity is due to changes in species composition or to reduced physiological abilities of the microbial community.In present study,the microbial biomass, phospholipid fatty acid(PLFA),community level catabolic profiles(CLCPs) and 16S rDNA-denaturing gradient gel electrophoresis(DGGE) of microbial community in surface soils accumulating heavy metal copper ion were investigated.In copper sulfate input soils,microbial biomass was reduced by 16.7%.Furthermore,PLFA analysis showed that the biomass of gram positive(G+) bacteria and fungi was restrained remarkably by 27.3% and 57.6%.,respectively.The analysis of CLCPs showed that both normal and high copper sulfate inputs significantly increased catabolic activity of G+ bacteria.DGGE analysis indicated that the genetic diversity of bacterial communities decreased in copper sulfate contaminated soils.Among 26 sequenced bands original from the culture medium of Biolog microplate and soil samples,20 of them were uncultivable bacteria,some G-bacteria resistant to copper ion,including actinobacterium,pseudomonas and proteobacterium,have been detected.In summary,we found that high dosage of copper sulfate had serious negative influence on soil microbial community,and normal dosage also had some adverse affects on fungi and part of G- bacteria.Our work demonstrated that copper sulfate imposed its impacts on soil microbial community through inhibiting fungi and G+ bacteria but promoting part of G- bacteria.

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