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《江苏省遗传学会第八届会员代表大会暨学术研讨会论文集》2010年
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Differential responses of the diazotrophic community to aluminum-tolerant and -sensitive soybean genotypes in acidic soil

【摘要】:正Phytotoxic aluminum(Al) is one of the most serious limiting factors for crop production in acidic soil.With the function of biological N fixation,the soybean could be considered as an excellent rotation and intercropping crop for improving soil fertility and structure.The biological nitrogen fixation is carried out by prokaryotic organisms harboring the nitrogenase enzyme encoded by the nif,anf or vnf genes.The nifH gene encodes the iron protein subunit of nitrogenase,and a good correlation between the nifH gene and 16S rRNA gene phylogeny has been found for diazotrophic microorganisms.However,using the nifH gene as a molecular marker in natural environments can be seen as an advantage,as it provides evidence for potential nitrogen fixation.The mechanism of plant Al tolerance has been generally studied in relation to the exudation of organic compounds chelating Al into non-rhizotoxic complexes in apoplast and symplast.Two contrasting soybean genotypes[BX10(Al-tolerant) and BD2(Al-sensitive)]were previously characterized for root exudation and formation of oxalate,malate and citrate and their related enzyme activities in response to -P,+Al,or -P and +Al combined.To characterize the responses of the diazotrophic community to soybean genotypes under Al toxicity, the diversity and abundance of nifH gene were assessed in the rhizosphere soils of BX10 and BD2,at different growth stages.As a result,changes of the community structure,determined by denaturing gradient gel electrophoresis(DGGE),were associated with genotypes at day 40 and the presence of soybean at days 76 and 107.Phylogenetic analyses indicated two and twelve nonidentical sequences were responsive to BX10 and BD2 during the whole growth periods,respectively,and some of sequences in BD2 affiliated with Rhizobium sp.and Azospirillum brasilens with potential in promoting plant growth.In addition,dominant diazotrophs varied with the soybean growth.NifH gene abundance determined by real-time PCR was higher in BX10 than BD2 at days 76 and 107.Furthermore,the community structure in BD2 was responsive to phytotoxic Al stress at day 40, while this was not the case for BX10 by canonical correspondence analysis(CCA) results.These results suggest that the diazotrophic community displayed different responses in the two soybean genotypes adapted to Al stress, characterized by increasing the quantity of diazotrophs in BX10 and stimulating beneficial diazotrophic bacteria in BD2.
【分类号】:S565.1

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