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《中国生物工程学会第二届青年科技论坛暨首届青年工作委员会学术年会论文集》2017年
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The genome sequence of Chinese Herb Erigeron breviscapus and complete biosynthesis of scutellarin in yeast

Huifeng Jiang  
【摘要】:Erigeron breviscapus is a well-known traditional Chinese medicinal herb to treat cardiovascular and cerebrovascular diseases. Scutellarin is the main active ingredient of E. breviscapus. Farming supply of E. breviscapus resource is not enough to satisfy the requirement of increasing markets in China. A whole-genome sequencing of E. breviscapus, leading to a preliminary draft of the genome, was combined with bioinformatics analysis, enzyme discovery, pathway construction and strain optimization to realize full scutellarin biosynthesis in yeast starting from glucose. Two key enzymes Flavonoids 6-hydroxylase(F6H) and Flavonoid 7-O-glucuronosyltransferase(F7GAT) in the pathway were identified from E. breviscapus for the first time. After metabolic engineering and fed-batch fermentation, the final yields of scutellarin and another active ingredient apigenin-7-O-glucuronide respectly reached to 108 and 185 mg/L after the fed-batch fermentation. Our research realizes an alternative supply for these medically relevant compounds and will bring this traditional Chinese medicine benefit to the cerebrovascular patients around the world.
【作者单位】:Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
【分类号】:S567;Q943.2
【正文快照】:
The genome sequence of Chinese Herb Erigeron breviscapus and complete biosynthesis of scutellarin in yeast@Huifeng Jiang$Tianjin Institute of Industrial Biotechnology, Chinese Academy of SciencesErigeron breviscapus is a well-known traditional Chinese me

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