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Endophytic fungi JXP25 extracts from Dalbergia odorifera T. Chen inhibits the growth of Staphylococcus aureus by damaging the cell membrane

Yuan Gao  Xiaomeng Zhang  Xue liu  Junhan Guo  Lijun Yan  
【摘要】:This study investigated the antibacterial effects of the endophytic fungi from Dalbergia odorifera T.Chen and the mechanism on Staphylococcus aureus.JXP25 were isolated from bark of Dalbergia odorifera T.Chen and identified by amplification of ITS rRNA gene sequence.The antibacterial activity was determined by measuring the minimum inhibitory concentration(MIC),minimum bactericidal concentration(MBC),and the time-kill assay.Results showed that endophytic fungi products can inhibit the growth of 5 pathogenic bacteria and the most susceptive strain was S.aureus with the lowest MIC and MBC values of 0.13 and 0.50 mg/mL,respectively.The membrane permeability study was determined by DNA leakage assay and electric conductivity assay.Furthermore the whole-cell protein lysates and DNA fragmentation assay were evaluated.The results indicated the DNA and protein of S.aureus was decreased gradually with the increase of endophytic fungi products' concentration.The probable antibacterial mechanism of endophytic gungi products was the change in permeability and integrity of the disrupted cell membranes,resulting in leakage of nucleic acids and proteins.This study provides new insights for understanding the antibacterial actions of endophytic fungi products for biomedical applications.

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