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The Mechanism of Ganoderma lucidum Specific microRNA glu-miR-01Regulation of Target Genes on PI3K/AKT/mTOR Signaling Pathway

Shouliang Li  Xiaoyuan Xu  Tianlong Jiang  Chunxin Xia  Huaixiang Zhou  Qing Sheng  
【摘要】:Ganoderma lucidum is a rare Chinese traditional medicine which is beneficial to the mind and body,prolonging life used by Chinese medicine.Our previous study found that Ganoderma lucidum miR-01 entered mice by oral administration and up-regulated expression in 293 T cells and affected the expression of mTOR protein.In addition,bioinformatics predicted that Ganoderma lucidum miR-01 could target key genes in the human PI3 K/AKT/mTOR signaling pathway.On the basis of this discovery,we propose the hypothesis that Ganoderma lucidum specific miRNA can regulate PI3 K/AKT/mTOR signaling pathway by acting on its target genes.This project will utilize dual-luciferase reporter gene assay system to determine Ganoderma lucidum specific miRNA miR-01 targeted genes involving PI3 K/AKT/mTOR signaling pathway.Furthermore,miRNA overexpression and RNA interference techniques will be used with FACS,LSCM detection and Immunohistochemistry,etc.In our experiments,to explore the mechanisms of miR-01 regulating PI3 K/AKT/mTOR signaling pathway.This study will provide a new way for deep cognition on the unique efficacy and transformation application of Ganoderma lucidum.

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