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《中国生物化学与分子生物学会第十一次会员代表大会暨2014年全国学术会议论文集——青年科学家论坛》2014年
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Pachytene piRNAs Instruct Massive mRNA Elimination during LateSpermiogenesis

Lan-Tao Gou  Peng Dai  Jian-Hua Yang  En-Duo Wang  Mo-Fang Liu  
【摘要】:Spermatogenesis in mammals is characterized by two waves of piRNA expression:one correspondsto classic piRNAs responsible for silencing retrotransponsons and the secondwave is predominantly derived from non-transposon intergenic regions in pachytene spermatocytes,but the function of these pachytene piRNAs is largely unknown.Here,we reportthe involvement of pachytene piRNAs in instructing massive mRNA elimination inmouse elongating spermatids.We demonstrate that a piRNA-induced silencing complex(pi-RISC)containing murine PIWI(MIWI)and deadenylase CAF1 is selectively assembled inelongating spermatids,which is responsible for inducing mRNA deadenylation and decayvia a mechanism that resembles the action of miRNAs in somatic cells.Such a highly orchestratedprogram appears to take full advantage of the enormous repertoire of diversifiedtargeting capacity of pachytene piRNAs derived from non-transposon intergenic regions.These findings suggest that pachytene piRNAs are responsible for inactivating vastcellular programs in preparation for sperm production from elongating spermatids.

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