Modular pathways for editing non-cognate amino acids by human cytoplasmic leucyl-tRNA synthetase

【摘要】：正To prevent potential errors in protein synthesis,some aminoacyl-tRNA synthetases have evolved editing mechanisms to hydrolyze misactivated amino acids(pretransfer editing) or misacylated tRNAs(post-transfer editing).Class la leucyl-tRNA synthetase(LeuRS) may misactivate various natural and non-protein amino acids and then mischarge tRNALeu.It is known that the fidelity of prokaryotic LeuRS depends on multiple editing pathways to clear the mis-intermediate and mis-products in the every steps of aminoacylation reaction.Here,we obtained human cytoplasmic LeuRS(hcLeuRS) and tRNALeu(hctRNALeu)