tRNA-dependent Pre-transfer editing by E.coli leucyl-tRNA synthetase

【摘要】：正Aminoacyl-tRNA synthetases(aaRSs) catalyze the covalent attachment of amino acids to their cognate tRNAs. Aminoacyiation errors lead to genetic code ambiguity and statistical proteins.Some synthetases evolved editng function to hydrolyze misactivated amino acids (pre-transfer editing) or mischanrged tRNAs (post-transfer editing).E coli Leucyl- tRNA synthetase (EcLeuRS) is thought to be relying solely on post-transfer editing to correct its mistakes (1).AN2090 is an