Chaperone effects upon prion propagation
【摘要】:正Ure2 is the protein determinant of the [URE3] prion phenotype in Saccharomyces cerevisiae and consists of a flexible N- terminal prion-determining domain and a globular C-terminal glutathione transferase-like domain.We have applied a range of biophysical techniques to study the mechanisms of folding,fibril assembly and enzymatic activity for the Ure2 protein [1].Genetic evidence suggests the involvement of molecular chaperones in propagation or curing of the [URE3] prion state