Amyloid nucleation and hierarchical assembly of Ure2p fibrils: Role of Asn/Gln repeat and non-repeat regions of the prion domain

【摘要】：正 The yeast protein Ure2 has prion characteristics in vivo and forms amyloid-likefibrils in vitro.The prion properties of these proteins are determined by theN-terminal region,which is rich in Asn and Gln,a motif thought to cause humandisease by forming stable protein aggregates.The Asn/Gln region of the Ure2p priondomain extends to residue 89,but residues 15 to 42 represent an island of‘normal’