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Lymphocyte proliferation triggered differentially by supernatants of mast cells activated with foot-and-mouth disease virus-like particles

Jiaxin Wang  Huiting Liang  Xiaoyun Tian  Ping Li  Luda Yang  Cheng Chang  Limin Li  
【摘要】:Foot-and-mouth disease virus(FMDV) is a highly contagious virus that can cause disease outbreaks in cloven-hoofed animals. Understanding the host-pathogen interaction and the contributions of innate versus adaptive immune responses has become a central topic in FMDV research. It has long been demonstrated that FMDV infects farm animals mainly through the mucosa, where mast cells distribute constitutively. However, the role of mast cells in fighting against FMDV remains unclear. We found that FMDV-like particles(VLP) could activate mast cells via toll-like receptor(TLR)2, TLR4, mannose receptor, and scavenger receptor, resulting in degranulation and cytokine secretion. To investigate the potential biology of VLP-activated mast cells on the proliferation of lymphocytes. The peritoneal mast cells(pMC) pool was randomly divided into three groups. The pMC pulsed with VLP were used as control, while the tanshinone Ⅱ A-pretreated pMC pulsed with VLP as test group, and pMC alone as blank group. After 6 h of VLP stimulation, the supernatants were collected from the different pMC culture wells. The pMC adherent to the coverslips were identified by toluidine blue staining. The pMC degranulation was substantially induced by VLP. Consequently, lymphocyte proliferation was differentially triggered by the supernatants of VLP-pulsed pMC. Of noting, lymphocyte proliferation was remarkably reduced in the present of supernatant from test group. Importantly, lymphocyte proliferation was significantly inhibited by supernatants collected from naive pMC and VLP-activated pMC. Surprisingly, T lymphocyte proliferation was very significantly inhibited by the supernatant from tanshinone Ⅱ A-pretreated pMC, while the granular components released from pMC pulsed with VLP are capable of stimulating the proliferation of splenic lymphocytes. By contrast, the secretory components of pMC loaded with VLP significantly inhibit splenic lymphocyte proliferation. To our best knowledge, this data for the first time reveal an unappreciated role of mast cells in the modulation of adaptive immunity to FMDV.

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