A STUDY ON THE REMINERALIZATION DURATION AFTER THE USE OF THE FLUORIDE TOOTHPASTE
【摘要】：正Objective:The aim of the study was to evaluate the remineralization effect on demineralized human enamel with different concentrations of fluoride solution(NaF)by confocal laser scanning microscope(CLSM)in vitro and to find the fluoride concentration on which level the fluoride solution had the remineralizaion effect on human enamel.The study also aimed to observe the fluoride concentrations in saliva at different time points after the use of fluoride toothpaste in order to investigate the remineralization duration in vivo. Methods:There were 5 groups in experiment l.The 9 human permanent teeth were dimineralized with pH4 gel for 96 hours.Each of these teeth were made into 5 enamel specimens.These 5 enamel specimens were randomly divided into 5 groups,according to the F~- level(ppm):0.01(group B),0.1(group C),l(group D),10(group E)and deionized water(group A)as control.The effect of remineralization was assessed with confocal laser scanning microscope(CLSM)by measuring three lesion parameters(area, total and average fluorescence).In experiment 2,Fluoride Ion Specific Electrode was used to measure the fluoride concentrations in non-irritating saliva of 22 healthy subjects before and after toothbrushing with fluoride toothpastes.All the data were analyzed with software SAS8.0. Results:There was significant difference between group A and C,A and D,A and E(P0.05),but no significant difference between group A and B(P0.05).The results showed that the fluoride concentration in saliva collect-ed lmin,0.5h,lh,2h and 3h after toothbrushing were significantly higher compared with the baseline,(P0.05).The average fluoride concentration on 3 hours after toothbrushing was higher than 0.1ppm. Conclusion:The minimum fluoride concentration is 0.1ppm on which level the fluoride solution has the remineralizaion effect on human enamel. The remineralization duration after the use of the fluoride toothpaste is 3 hours.