In vitro and in vivo study on antioxidant activities of Hemp seeds oil

【摘要】：正The present study was designed to evaluate the antioxidant activities of Hemp seeds oil(HSO ) in vivo and in vitro.DPPH radical scavenging system was adopted to evaluate the kinetic indexes of the methanol extracts from HSO.Different concentrations of HSO(1.365、2.73、8.19g/kgBW) were intragastrically administered to ICR mice for 30 days,the total body gamma irradiation(~(60)Co) was used to establish the mice model of peroxidation injury.Meanwhile the normal control group and irradiation model group without HSO interference were set.On the third day after irradiation,MDA contents and SOD activity in mouse serum and tissues were observed.Methanol extracts from HSO had strong effect against free radical in vitro.The intervention of HSO can effectively reduce the MDA contents and increase the SOD acvtivity in mice.Thus,our results show that HSO has strong antioxidant effect both in vitro and in vivo.