收藏本站
《科技引领产业、支撑跨越发展——第六届湖北科技论坛论文集萃》2011年
收藏 | 手机打开
二维码
手机客户端打开本文

The anti-tumor effects of flavonoids from the ethnic medicine Docycia delavayi(Franch.)Schneid.and its possible mechanism

Xukun Deng  Xiangpei Zhao  Lvyi Chen  Ze yang Zhou  Zhinan Mei  Qinxiong Lin  
【摘要】:正Docycia delavayi(Franch.) Schneid.,a Chinese Dai ethnic medicine popularly known as "Duoyi" in Yunnan province of China,had been traditionally used as a folk remedy for fever,cancer,fracture and beriberoid disease for a long time in China.The present study was carried out to investigate the active components and the anti -tumor effect mechanism of the flavonoids including chrysin,quercetin,naringenin and avicularin from Docycia delavayi(Franch.) Schneid..The MTT-assay showed that the inhibitory activity of chrysin was much stronger than the other three flavonoids on HepG2 cells,EC109 cells,Hela cells and SW480 cells for 48 h treatment in vitro,with the IC50 values of 73.7,85.1,114.7,106.3μM,respectively.Further study revealed that chrysin exhibited the strong suppressive effect on HepG2 in concentration- and time-dependent manners.In addition,chrysin caused HepG2 cell shrinkage,membrane blebbing,apoptotic body formation,all of which are typical characteristics of apoptotic programmed cell death.The results of flow cytometric analysis demonstrated that chrysin caused apoptosis of HepG2 cells through cell cycle arrest at G0/G1 phase,thus preventing cells entering S phase. Immunoblot results showed that chrysin significantly increased the expression levels of caspase-3 and Bax protein, and decreased the expression level of B-cell lymphoma/leukemia-2(Bcl-2) protein.These findings indicate that the major flavonoids present in Docycia delavayi(Franch.) Schneid.effectively inhibit the growth activity of tumor cells,among which chrysin introduce HepG2 cells death via apoptosis,probably through the participation of caspase-3,Bax and Bcl-2.

【相似文献】
中国重要会议论文全文数据库 前10条
1 Boris J.P.Kaus;;Thermal localization as a potential mechanism to rift cratons[A];中国科学院地质与地球物理研究所第11届(2011年度)学术年会论文集(上)[C];2012年
2 ;Novel mechanism of endothelial activation by shear stress[A];中国生理学会第23届全国会员代表大会暨生理学学术大会论文摘要文集[C];2010年
3 ;Mechanism of Benzene Hydroxylation by High-Valent Bare Fe~Ⅳ=O~(2+):Explicit Electronic Structure Analysis[A];中国化学会第28届学术年会第13分会场摘要集[C];2012年
4 ;Mechanism of Benzene Hydroxylation by High-Valent Bare Fe~Ⅳ=O~(2+):Explicit Electronic Structure Analysis[A];中国化学会第28届学术年会第8分会场摘要集[C];2012年
5 Stephen T.O'Rourke;;20-HETE increases NADPH oxidase-derived ROS production and stimulates L-type calcium channel via PKC-dependent mechanism in cardiomyocytes[A];中国生理学会第23届全国会员代表大会暨生理学学术大会论文摘要文集[C];2010年
6 Edward S.Yeung;;Probing the interaction mechanism of cell-penetrating peptides with model lipid membranes by total internal scattering microscopy[A];第十六届全国光散射学术会议论文摘要集[C];2011年
7 孙勇;文志斌;何晓凡;李邦涛;汤旭东;贺石林;;Influence of tetramethylpyrazine on the expression of tissuefactor induced by thrombin and its mechanism in vascularendothelial cells[A];湖南省生理科学会2006年度学术年会论文摘要汇编[C];2007年
8 ;Characterization of Ring1b,a possible E3 ligase of p53,in zebrafish early development and human testicular cells[A];2010·第一届斑马鱼学术研讨会论文摘要集[C];2010年
9 陈少瑾;陈宜菲;谢凝子;张二华;吴双桃;;土壤中硝基苯化合物被零价铁还原的机理[A];第三届全国环境化学学术大会论文集[C];2005年
10 ;Functional expression of large-conductance calcium-activated potassium channels in human endometrium:an possible mechanism involved in endometrial receptivity and embryo implantation[A];2011中国妇产科学术会议暨浙江省计划生育与生殖医学学术年会暨生殖健康讲习班论文汇编[C];2011年
中国重要报纸全文数据库 前2条
1 日本中央大学博士候补人 陈海权;中国入世后的战略构想[N];国际金融报;2002年
2 麻城二中 刘裕松;英语高考考前复习五要点[N];黄冈日报;2011年
中国博士学位论文全文数据库 前1条
1 哈桑(Hassan Yousefi Oderji);分子动力学研究流体的输运系数和纳米合金粒子的热融机理[D];大连理工大学;2012年
 快捷付款方式  订购知网充值卡  订购热线  帮助中心
  • 400-819-9993
  • 010-62791813
  • 010-62985026